Journal article
Usefulness of [18F]-DA and [18F]-DOPA for PET imaging in a mouse model of pheochromocytoma
Nuclear Medicine and Biology, Vol.39(2), pp.215-226
2012
Abstract
Purpose: To evaluate the usefulness of [ 18F]-6-fluorodopamine ([ 18F]-DA) and [ 18F]-L-6-fluoro-3,4-dihydroxyphenylalanine ([ 18F]-DOPA) positron emission tomography (PET) in the detection of subcutaneous (s.c.) and metastatic pheochromocytoma in mice; to assess the expression of the norepinephrine transporter (NET) and vesicular monoamine transporters 1 and 2 (VMAT1 and VMAT2), all important for [ 18F]-DA and [ 18F]-DOPA uptake. Furthermore, to compare tumor detection by micro-computed tomography (microCT) to magnetic resonance imaging (MRI) in individual mouse. Methods: SUV max values were calculated from [ 18F]-DA and [ 18F]-DOPA PET, tumor-to-liver ratios (TLR) were obtained and expression of NET, VMAT1 and VMAT2 was evaluated. Results: [ 18F]-DA detected less metastatic lesions compared to [ 18F]-DOPA. TLR values for liver metastases were 2.26-2.71 for [ 18F]-DOPA and 1.83-2.83 for [ 18F]-DA. A limited uptake of [ 18F]-DA was found in s.c. tumors (TLR=0.22-0.27) compared to [ 18F]-DOPA (TLR=1.56-2.24). Overall, NET and VMAT2 were expressed in all organ and s.c. tumors. However, s.c. tumors lacked expression of VMAT1. We confirmed [ 18F]-DA's high affinity for the NET for its uptake and VMAT1 and VMAT2 for its storage and retention in pheochromocytoma cell vesicles. In contrast, [ 18F]-DOPA was found to utilize only VMAT2. Conclusion: MRI was superior in the detection of all organ tumors compared to microCT and PET. [ 18F]-DOPA had overall better sensitivity than [ 18F]-DA for the detection of metastases. Subcutaneous tumors were localized only with [ 18F]-DOPA, a finding that may reflect differences in expression of VMAT1 and VMAT2, perhaps similar to some patients with pheochromocytoma where [ 18F]-DOPA provides better visualization of lesions than [ 18F]-DA.
Details
- Title
- Usefulness of [18F]-DA and [18F]-DOPA for PET imaging in a mouse model of pheochromocytoma
- Authors/Creators
- L. Martiniova (Author/Creator) - Eunice Kennedy Shriver National Institute of Child Health and Human DevelopmentS. Cleary (Author/Creator) - National Institute of Neurological Disorders and StrokeE.W. Lai (Author/Creator) - Eunice Kennedy Shriver National Institute of Child Health and Human DevelopmentD.O. Kiesewetter (Author/Creator) - National Institute of Biomedical Imaging and BioengineeringJ. Seidel (Author/Creator) - Molecular Imaging Program, National Cancer Institute, NIH, Bethesda, MD 20892, USAL.F. Dawson (Author/Creator) - Murdoch UniversityJ.K. Phillips (Author/Creator) - Murdoch UniversityD. Thomasson (Author/Creator) - National Institutes of HealthX. Chen (Author/Creator) - National Institute of Biomedical Imaging and BioengineeringG. Eisenhofer (Author/Creator) - Dresden University of TechnologyJ.F. Powers (Author/Creator) - Tufts Medical CenterR. Kvetnansky (Author/Creator) - Institute of Experimental Endocrinology of the Slovak Academy of SciencesK. Pacak (Author/Creator) - Institute of Experimental Endocrinology of the Slovak Academy of Sciences
- Publication Details
- Nuclear Medicine and Biology, Vol.39(2), pp.215-226
- Publisher
- Elsevier BV
- Identifiers
- 991005540336607891
- Copyright
- © 2012 Elsevier Inc.
- Murdoch Affiliation
- School of Veterinary and Biomedical Sciences
- Language
- English
- Resource Type
- Journal article
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