Journal article
Using integrated omics to assess the effects of rhinovirus infection in children with Cystic Fibrosis (CF)
Respiratory infections, Vol.56(Supp. 64), Art. 4342
2020
Abstract
Introduction/Aim: Children with Cystic Fibrosis (CF) typically exhibit prolonged and severe symptoms during rhinovirus (RV) infection compared to healthy children. Here, we studied the host-rhinovirus interaction signature after infection, integrating two omics: (1) Whole transcriptome sequencing (WTS) to determine the viral load and host’s gene expression, and (2) metabolomics to profile the metabolites associated with the viral infection of primary tracheal epithelial cells obtained from healthy children (H) and those with CF.
Methods: WTS and hydrophilic interaction liquid chromatography (HILIC) coupled to mass spectrometry, were used to identify the differences in transcripts/metabolites and RV coverage produced by H (3.9 ± 1.5 years; n=8) and CF (2.6 ± 1.8 years; n=8; all p.Phe508del/p.Phe508del) primary epithelial cells at 24 hours post-infection with human rhinovirus 1B. Univariate and multivariate analyses were then performed to identify the infection hallmark.
Results: RV coverage in uninfected cells (Mock) was less than 0.5X, whereas, infected cells presented 44.4X and 101.6X of RV in children with and without CF respectively. Global RV infection was associated with 14 metabolites and 1713 genes differential expressed. From these, several metabolic pathways were found dysregulated including inositol metabolism and glycerophospholipid biosynthesis.
Conclusion: Metabolic host-derived pathways associated with RV infection were identified. Although functional analysis is still required, these pathways could be used as potential biomarkers of RV infection in CF. Future analysis will help to understand whether these compounds can be targeted for antiviral purposes.
Details
- Title
- Using integrated omics to assess the effects of rhinovirus infection in children with Cystic Fibrosis (CF)
- Authors/Creators
- P. Agudelo-Romero (Author/Creator) - The Kids Research Institute AustraliaK. Martinovich (Author/Creator) - The Kids Research Institute AustraliaJ.D. Chandler (Author/Creator) - Emory UniversityK-M Ling (Author/Creator) - The Kids Research Institute AustraliaE.N. Sutanto (Author/Creator) - The Kids Research Institute AustraliaD.P. Jones (Author/Creator) - Emory UniversityR.M. Tirouvanzium (Author/Creator) - Emory UniversityS.M. Stick (Author/Creator) - The University of Western AustraliaL.W. Garratt (Author/Creator) - The Kids Research Institute AustraliaA. Kicic (Author/Creator) - The University of Western AustraliaE. Wa (Author/Creator)C.F. Arest (Author/Creator)
- Publication Details
- Respiratory infections, Vol.56(Supp. 64), Art. 4342
- Publisher
- European Respiratory Society
- Identifiers
- 991005544088307891
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
- Additional Information
- This abstract was presented at the 2020 ERS International Congress, in session “Respiratory viruses in the "pre COVID-19" era”.
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- Web Of Science research areas
- Respiratory System
- ESI research areas
- Clinical Medicine