Journal article
Using synthetic oligonucleotides as standards in probe-based qPCR
BioTechniques, Vol.64(4), pp.177-179
2018
Abstract
Real-time PCR (qPCR) is widely used in the life sciences. For quantifying DNA, a standard curve is required. Common methods for standard development are time consuming, costly, necessitate a specific skill set, and pose a contamination risk. Using a targeted synthetic oligonucleotide, such as a gBlocks® Gene Fragment, overcomes these drawbacks and provides researchers an accurate and quick solution to standard development. Here, we demonstrate that using a gBlocks fragment as a standard provides comparable sensitivity, reliability, and assay performance to a purified amplicon standard.
Details
- Title
- Using synthetic oligonucleotides as standards in probe-based qPCR
- Authors/Creators
- J. Conte (Author/Creator) - Keystone CollegeM.J Potoczniak (Author/Creator) - Arcadia UniversityS.S. Tobe (Author/Creator) - Arcadia University
- Publication Details
- BioTechniques, Vol.64(4), pp.177-179
- Publisher
- Informa BioSciences
- Identifiers
- 991005540668207891
- Copyright
- © 2018 Future Science Group
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
133 File views/ downloads
98 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.54 Molecular & Cell Biology - Genetics
- 1.54.1454 PCR Techniques
- Web Of Science research areas
- Biochemical Research Methods
- Biochemistry & Molecular Biology
- ESI research areas
- Biology & Biochemistry