Utilization of low-molecular-weight organic compounds by the filterable fraction of a lotic microbiome

Lydia-Ann J Ghuneim; Marco A Distaso; Tatyana N Chernikova; Rafael Bargiela; Evgenii A Lunev; Aleksei A Korzhenkov; Stepan V Toshchakov; David Rojo; Coral Barbas; Manuel Ferrer; Olga V Golyshina; Peter N Golyshin; David L Jones

doi:10.1093/femsec/fiaa244

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$Utilization of low-molecular-weight organic compounds by the filterable fraction of a lotic microbiome$

Journal article

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Utilization of low-molecular-weight organic compounds by the filterable fraction of a lotic microbiome

Lydia-Ann J Ghuneim, Marco A Distaso, Tatyana N Chernikova, Rafael Bargiela, Evgenii A Lunev, Aleksei A Korzhenkov, Stepan V Toshchakov, David Rojo, Coral Barbas, Manuel Ferrer, …

FEMS microbiology ecology, Vol.97(2), fiaa244

2021

DOI: https://doi.org/10.1093/femsec/fiaa244

PMID: 33264383

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Abstract

Carbon

Fresh Water

Microbiota

Organic Chemicals

Rivers

RNA, Ribosomal, 16S - genetics

Filterable microorganisms participate in dissolved organic carbon (DOC) cycling in freshwater systems, however their exact functional role remains unknown. We determined the taxonomic identity and community dynamics of prokaryotic microbiomes in the 0.22 µm-filtered fraction and unfiltered freshwater from the Conwy River (North Wales, UK) in microcosms and, using targeted metabolomics and 14C-labelling, examined their role in the utilization of amino acids, organic acids and sugars spiked at environmentally-relevant (nanomolar) concentrations. To identify changes in community structure, we used 16S rRNA amplicon and shotgun sequencing. Unlike the unfiltered water samples where the consumption of DOC was rapid, the filtered fraction showed a 3-day lag phase before the consumption started. Analysis of functional categories of clusters of orthologous groups of proteins (COGs) showed that COGs associated with energy production increased in number in both fractions with substrate addition. The filtered fraction utilized low-molecular-weight (LMW) DOC at much slower rates than the whole community. Addition of nanomolar concentrations of LMW DOC did not measurably influence the composition of the microbial community nor the rate of consumption across all substrate types in either fraction. We conclude that due to their low activity, filterable microorganisms play a minor role in LMW DOC processing within a short residence time of lotic freshwater systems.

Details

Title: Utilization of low-molecular-weight organic compounds by the filterable fraction of a lotic microbiome
Authors/Creators: Lydia-Ann J Ghuneim - Bangor University
Marco A Distaso - Bangor University
Tatyana N Chernikova - Bangor University
Rafael Bargiela - Bangor University
Evgenii A Lunev - Immanuel Kant Baltic Federal University
Aleksei A Korzhenkov - Kurchatov Institute
Stepan V Toshchakov - Czech Academy of Sciences, Institute of Microbiology
David Rojo - Universidad San Pablo CEU
Coral Barbas - Universidad San Pablo CEU
Manuel Ferrer - Institute of Catalysis, Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain.
Olga V Golyshina - Bangor University
Peter N Golyshin - Bangor University
David L Jones - UWA School of Agriculture and Environment, The University of Western Australia, Perth, WA 6009, Australia
Publication Details: FEMS microbiology ecology, Vol.97(2), fiaa244
Publisher: Oxford University Press on behalf of FEMS.
Identifiers: 991005560328307891
Murdoch Affiliation: Centre for Sustainable Farming Systems
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.83 Bioengineering; 3.83.167 Microbial Diversity
Web Of Science research areas: Microbiology
ESI research areas: Microbiology