Journal article
Vaccine-Induced Allo-HLA–Reactive Memory T Cells in a Kidney Transplantation Candidate
Transplantation, Vol.91(6), pp.645-651
2011
Abstract
Background: Allo-human leukocyte antigen (HLA) reactivity by naturally acquired viral-specific memory T cells is common. However, the effect of successful vaccination on the alloreactive memory T-cell repertoire is unclear. We hypothesized that vaccination could specifically induce allo-HLA–reactive memory T cells.
Methods: A varicella-zoster virus (VZV) immediate early 62 (IE62)-specific CD8 memory T-cell clone was single cell sorted from a VZV seronegative renal transplant candidate after response to live attenuated varicella vaccination. To analyze the allo-HLA reactivity, the VZV IE62-specific T-cell clone was tested against HLA-typed target cells and target cells transfected with HLA molecules, in both cytokine production and cytotoxicity assays.
Results: The varicella vaccine–induced VZV IE62-specific T-cell clone specifically produced interferon-γ when stimulated with HLA-B*55:01–expressing Epstein-Barr virus–transformed B cells and HLA-B*55:01–transfected K562 cells (single HLA antigen expressing cell line [SALs]) only. The clone also demonstrated specific cytolytic effector function against HLA-B*55:01 SALs and phytohemagglutinin blasts. Cytotoxicity assays using proximal tubular epithelial cell and human umbilical vein endothelial cell targets confirmed the kidney tissue specificity of the allo-HLA-B*55:01 reactivity, and the relevance of the cross-reactivity to clinical kidney transplantation. The results also suggest that molecular mimicry, and not bystander proliferation, is the mechanism underlying vaccine-induced alloreactivity.
Conclusions: Varicella vaccination generated a de novo alloreactive kidney cell–specific cytolytic effector memory T cell in a patient awaiting renal transplantation. Vaccination-induced alloreactivity may have important clinical implications, especially for vaccine timing and recipient monitoring.
Details
- Title
- Vaccine-Induced Allo-HLA–Reactive Memory T Cells in a Kidney Transplantation Candidate
- Authors/Creators
- L.J.A. DʼOrsogna (Author/Creator)N.M. van Besouw (Author/Creator)E.M.W. van der Meer-Prins (Author/Creator)P. van der Pol (Author/Creator)M. Franke-van Dijk (Author/Creator)Y.M. Zoet (Author/Creator)A. van der Slik (Author/Creator)W. Weimar (Author/Creator)C. van Kooten (Author/Creator)A. Mulder (Author/Creator)D.L. Roelen (Author/Creator)I.I.N. Doxiadis (Author/Creator)F.H.J. Claas (Author/Creator)
- Publication Details
- Transplantation, Vol.91(6), pp.645-651
- Publisher
- Wolters Kluwer Health
- Identifiers
- 991005542791507891
- Copyright
- © 2017 Wolters Kluwer Health, Inc.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.6 Immunology
- 1.6.127 T Cell Regulation
- Web Of Science research areas
- Immunology
- Surgery
- Transplantation
- ESI research areas
- Clinical Medicine