Validation of a LC-MS/MS method to simultaneously quantify thiafentanil and naltrexone in plasma for pharmacokinetic studies in wildlife

Judith T Christie; Mieghan Bruce; Silke Pfitzer; Liesel Laubscher; Jacobus P Raath; Michael Laurence; Tracy Kellermann

doi:10.1016/j.jchromb.2023.123990

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Validation of a LC-MS/MS method to simultaneously quantify thiafentanil and naltrexone in plasma for pharmacokinetic studies in wildlife

Journal article

Peer reviewed

Validation of a LC-MS/MS method to simultaneously quantify thiafentanil and naltrexone in plasma for pharmacokinetic studies in wildlife

Judith T Christie, Mieghan Bruce, Silke Pfitzer, Liesel Laubscher, Jacobus P Raath, Michael Laurence and Tracy Kellermann

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, Vol.1233, 123990

2023

DOI: https://doi.org/10.1016/j.jchromb.2023.123990

PMID: 38190774

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Abstract

LC-MS/MS

Thiafentanil

Naltrexone

Pharmacokinetics

Veterinary anaesthesia

Chemical immobilisation

Thiafentanil is a popular opioid agonist that is fully reversed by administering naltrexone. This agonist-antagonist combination is administered to a wide variety of wildlife species for chemical immobilisation, however plasma concentrations for thiafentanil remain unreported. This report describes a method that was developed and validated using human plasma and cross-validated for the analysis of goat plasma. Samples were extracted using a simple protein precipitation and analysed using LC-MS/MS. The assay was validated over the calibration range 4.38 - 1120 ng/mL for thiafentanil and 15.63 - 4000 ng/mL for naltrexone. The mean inter-day accuracies for QCs prepared in human plasma (n = 18) ranged from 94.8 - 103.8 % for thiafentanil and 94.8 - 95.9 % for naltrexone with corresponding precisions of 3.4 - 7.9 % and 2.8 - 11.4 %, respectively. The mean accuracies for QCs prepared in goat plasma (n = 6) ranged from 89.0 - 100.5 % for thiafentanil and 89.0 - 98.0 % for naltrexone with the associated precisions ranging from 7.1 - 11.6 % and 4.8 - 12.3 %, respectively. Both analytes were stable on bench for six hours and for three freeze-thaw cycles. The impact of heat-inactivation, necessary for the inactivation of potential foot-and-mouth disease, on analyte stability, matrix effect and recovery were evaluated, and a correction factor was established to determine the original analyte concentrations. The method was applied to pharmacokinetic samples collected from goats. The use of goats as a model species provides the first insight into the plasma concentrations of thiafentanil.

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Title: Validation of a LC-MS/MS method to simultaneously quantify thiafentanil and naltrexone in plasma for pharmacokinetic studies in wildlife
Authors/Creators: Judith T Christie - School of Veterinary Medicine, Harry Butler Institute, Murdoch University, Murdoch, Western Australia, Australia. Electronic address: 33913968@student.murdoch.edu.au
Mieghan Bruce - Murdoch University, Centre for Biosecurity and One Health
Silke Pfitzer - Tshwane University of Technology
Liesel Laubscher - Wildlife Pharmaceuticals, Rocky Drift, White River, South Africa
Jacobus P Raath - Wildlife Pharmaceuticals, Rocky Drift, White River, South Africa
Michael Laurence - School of Veterinary Medicine, Harry Butler Institute, Murdoch University, Murdoch, Western Australia, Australia
Tracy Kellermann - Stellenbosch University
Publication Details: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, Vol.1233, 123990
Publisher: Elsevier B.V.
Identifiers: 991005626968007891
Murdoch Affiliation: School of Veterinary Medicine
Language: English
Resource Type: Journal article

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