Journal article
Validation of a minimal panel of antibodies for the diagnosis of malignant pleural mesothelioma
Pathology, Vol.43(4), pp.313-317
2011
Abstract
AIMS:
We previously established the use of a minimal panel of antibodies as sufficient to diagnose most epithelial malignant mesothelioma (MPM). We aimed to validate this approach and investigate the utility of a D2-40 antibody.
METHODS:
A series of 80 MPM patients selected for surgery and 21 consecutive patients with pleural metastatic carcinoma were included. A minimal panel of antibodies, consisting of calretinin, BG8 and CD15, and D2-40 was investigated.
RESULTS:
There were 61 epithelial and 19 biphasic MPM as well as 12 metastatic lung, six breast (5 ductal adenocarcinomas, 1 mixed ductal/lobular adenocarcinoma), two serous papillary ovarian carcinomas and one moderately differentiated colorectal adenocarcinoma. The sensitivity of positive calretinin labelling to confirm the diagnosis of MPM was 97.5%, while the 'diagnostic sensitivities' of lack of labelling for BG8 and CD15 were 91.3% and 97.5%, respectively. The use of calretinin, BG8 and CD15 resulted in correct classification in 97.5% of all MPMs. All MPM cases investigated showed at least focal positive D2-40 labelling.
CONCLUSIONS:
We have validated the usefulness of a minimal panel of antibodies with calretinin, BG8 and CD15 as the initial step to the diagnosis of MPM. D2-40 emerged as a helpful diagnostic tool for cases where our initial approach failed to conclusively diagnose MPM.
Details
- Title
- Validation of a minimal panel of antibodies for the diagnosis of malignant pleural mesothelioma
- Authors/Creators
- S.C-H Kao (Author/Creator)K. Griggs (Author/Creator) - Flinders UniversityK. Lee (Author/Creator) - Concord Repatriation General HospitalN. Armstrong (Author/Creator) - Garvan Institute of Medical ResearchS. Clarke (Author/Creator) - The University of SydneyJ. Vardy (Author/Creator) - The University of SydneyN. van Zandwijk (Author/Creator) - Asbestos Diseases Research InstituteJ. Burn (Author/Creator) - South Australia PathologyB.C. McCaughan (Author/Creator) - Royal Prince Alfred HospitalD.W. Henderson (Author/Creator) - Flinders Medical CentreS. Klebe (Author/Creator) - Flinders Medical Centre
- Publication Details
- Pathology, Vol.43(4), pp.313-317
- Publisher
- Lippincott Williams and Wilkins
- Identifiers
- 991005543713407891
- Copyright
- © 2011 Royal College of Pathologists of Australasia
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
30 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.297 Asbestos & Mesothelioma
- 1.297.780 Asbestos-Mesothelioma Link
- Web Of Science research areas
- Pathology
- ESI research areas
- Clinical Medicine