Vitamin E and omega-3 fatty acids independently attenuate plasma concentrations of proinflammatory cytokines and prostaglandin E in lipopolysaccharide-challenged growing–finishing pigs

S.D. Upadhyaya; J.C. Kim; B.P. Mullan; J.R. Pluske; I.H. Kim

doi:10.2527/jas.2014-8330

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Vitamin E and omega-3 fatty acids independently attenuate plasma concentrations of proinflammatory cytokines and prostaglandin E in lipopolysaccharide-challenged growing–finishing pigs

Journal article

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Vitamin E and omega-3 fatty acids independently attenuate plasma concentrations of proinflammatory cytokines and prostaglandin E in lipopolysaccharide-challenged growing–finishing pigs

S.D. Upadhyaya, J.C. Kim, B.P. Mullan, J.R. Pluske and I.H. Kim

Journal of Animal Science, Vol.93(6), pp.2926-2934

2015

DOI: https://doi.org/10.2527/jas.2014-8330

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Abstract

This study tested the hypothesis that vitamin E (Vit E) and omega-3 fatty acids will additively attenuate the production of proinflammatory cytokines and PGE2 in immune system–stimulated growing–finishing pigs. A total of 80 mixed sex pigs weighing 50.7 ± 0.76 kg (mean ± SE) were blocked and stratified based on sex and BW to a 2 × 2 factorial design with the respective factors being 1) without and with 300 IU Vit E and 2) without and with 25% replacement of tallow to linseed oil as a source of n-3 fatty acids. Each treatment consisted of 4 replicate pens with 5 pigs (3 barrows and 2 gilts) per pen. All pigs were challenged with an intramuscular injection of Escherichia coli lipopolysaccharide (LPS; O111:B4) twice weekly over the 6-wk experiment. After LPS challenge, pigs fed a diet supplemented with n-3 fatty acids had fewer (P < 0.05) white blood cells and tended to show both a reduced (P < 0.10) proportion of lymphocytes and IgG concentration compared with pigs fed a diet without any supplements. Supplementation of n-3 fatty acids reduced (P < 0.01 and P < 0.05) serum concentrations of cortisol and tumor necrosis factor α (TNF-α), respectively. The serum concentration of PGE2 was decreased (P < 0.05) with supplementation of both Vit E and n-3 fatty acids; however, the extent of the reduction was greater (P < 0.001) in pigs fed an n-3 fatty acid–supplemented diet. However, there were no additive effects of the combined supplementation of Vit E and n-3 fatty acids on serum concentrations of proinflammatory cytokines and PGE2. The results suggest that n-3 fatty acids independently attenuate production of TNF-α and PGE2 in immune system–stimulated growing–finishing pigs.

Details

Title: Vitamin E and omega-3 fatty acids independently attenuate plasma concentrations of proinflammatory cytokines and prostaglandin E in lipopolysaccharide-challenged growing–finishing pigs
Authors/Creators: S.D. Upadhyaya (Author/Creator)
J.C. Kim (Author/Creator) - Agriculture and Food
B.P. Mullan (Author/Creator) - Agriculture and Food
J.R. Pluske (Author/Creator) - Murdoch University
I.H. Kim (Author/Creator) - Dankook University
Publication Details: Journal of Animal Science, Vol.93(6), pp.2926-2934
Publisher: American Society of Animal Science
Identifiers: 991005540543607891
Murdoch Affiliation: School of Veterinary and Life Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.51 Dairy & Animal Sciences; 3.51.208 Poultry Nutrition
Web Of Science research areas: Agriculture, Dairy & Animal Science
ESI research areas: Plant & Animal Science