Journal article
Volume of fluid and concentration of cations and energy substrates in the uteri of mice during early pseudopregnancy
Reproduction, Fertility and Development, Vol.1(2), pp.171-178
1989
Abstract
Estimates of the volume of fluid and the concentration of cations in the uterus were made by flushing the uterine cavity of mice mated to vasectomized males and measuring the concentrations of sodium and potassium cations in the recovered fluid. On day 1 following mating, large volumes of fluid (>90 fiL) were found in the uterus but, from days 2 to 5, only 2-5 /iL of fluid were present. The ratio of sodium to potassium fell from 4-5:1 on day 1 to 1-8:1 on days 2 to 5 of pseudopregnancy, indicating that uterine fluid in the mouse has a high K+ content ranging from 35 mEq L-1 on day 1 to 75 mEq L_1 on day 5. Glucose, lactate and pyruvate in uterine flushings were also assayed and their concentration in uterine fluid calculated using the volumes found above. The level of all substrates was low on day 1 after mating. From day 2 onwards approximately 1 mM glucose was present in the fluids. The concentration of lactate was more variable, and peaked at 4 mM on day 2 of pseudopregnancy. In general, the concentration of pyruvate was 10% of the lactate value.
Details
- Title
- Volume of fluid and concentration of cations and energy substrates in the uteri of mice during early pseudopregnancy
- Authors/Creators
- R.G. Wales (Author/Creator) - Murdoch UniversityW.R. Edirisinghe (Author/Creator)
- Publication Details
- Reproduction, Fertility and Development, Vol.1(2), pp.171-178
- Publisher
- CSIRO Publishing
- Identifiers
- 991005540547507891
- Copyright
- © CSIRO 1989
- Murdoch Affiliation
- School of Veterinary Studies
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 1 Clinical & Life Sciences
- 1.81 Reproductive Biology
- 1.81.339 Embryo Development
- Web Of Science research areas
- Developmental Biology
- Reproductive Biology
- Zoology
- ESI research areas
- Plant & Animal Science