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Journal article
Water-soluble microencapsulation using gum Arabic and skim milk enhances viability and efficacy of Pediococcus acidilactici probiotic strains for application in broiler chickens
Animal bioscience, Vol.37(8), pp.1440-1451
2024
PMID: 38575120
Abstract
Objective
This study aimed to develop and evaluate the effectiveness of a water-soluble microencapsulation method for probiotic strains using gum Arabic (GA) and skim milk (SKM) over a three-month storage period following processing.
Methods
Four strains of Pediococcus acidilactici (BYF26, BYF20, BF9, and BF14) that were typical lactic acid bacteria (LAB) isolated from the chicken gut were mixed with different ratios of GA and SKM as coating agents before spray drying at an inlet temperature 140°C. After processing, the survivability and probiotic qualities of the strains were assessed from two weeks to three months of storage at varied temperatures, and de-encapsulation was performed to confirm the soluble properties. Finally, the antibacterial activity of the probiotics was assessed under simulated gastrointestinal conditions.
Results
As shown by scanning electron microscopy, spray-drying produced a spherical, white-yellow powder. The encapsulation efficacy (percent) was greatest for a coating containing a combination of 30% gum Arabic: 30% skim milk (w/v) (GA:SKM30) compared to lower concentrations of the two ingredients (p<0.05). Coating with GA:SKM30 (w/v) significantly enhanced (p<0.05) BYF26 survival under simulated gastrointestinal conditions (pH 2.5 to 3) and maintained higher survival rates compared to non-encapsulated cells under an artificial intestinal juices condition of pH 6. De-encapsulation tests indicated that the encapsulated powder dissolved in water while keeping viable cell counts within the effective range of 106 for 6 hours. In addition, following three months storage at 4°C, microencapsulation of BYF26 in GA:SKM30 maintained both the number of viable cells (p<0.05) and the preparation’s antibacterial efficacy against pathogenic bacteria, specifically strains of Salmonella.
Conclusion
Our prototype water-soluble probiotic microencapsulation GA:SKM30 effectively maintains LAB characteristics and survival rates, demonstrating its potential for use in preserving probiotic strains that can be used in chickens and potentially in other livestock.
Details
- Title
- Water-soluble microencapsulation using gum Arabic and skim milk enhances viability and efficacy of Pediococcus acidilactici probiotic strains for application in broiler chickens
- Authors/Creators
- Ratchanida Kamwa - Chulalongkorn UniversityBenjamas Khurajog - Chulalongkorn UniversityNongnut Muangsin - Chulalongkorn UniversityPawiya Pupa - Chulalongkorn UniversityDavid John Hampson - Department of Biology, Faculty of Science, Chulalongkorn University, Bangkok 10330, ThailandNuvee Prapasarakul - Chulalongkorn University
- Publication Details
- Animal bioscience, Vol.37(8), pp.1440-1451
- Publisher
- Asian-Australasian Association of Animal Production Societies
- Identifiers
- 991005687469207891
- Copyright
- © 2024 by Animal Bioscience
- Murdoch Affiliation
- School of Veterinary Medicine
- Language
- English
- Resource Type
- Journal article
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