Journal article
Weakly haemolytic variants of Brachyspira hyodysenteriae newly emerged in Europe belong to a distinct subclade with unique genetic properties
Veterinary Research Communications, Vol.50, Article number: 21
2019
Abstract
Brachyspira (B.) hyodysenteriae is widespread globally, and can cause mucohaemorrhagic colitis (swine dysentery, SD) with severe economic impact in infected herds. Typical strains of B. hyodysenteriae are strongly haemolytic on blood agar, and the haemolytic activity is believed to contribute to virulence in vivo. However, recently there have been reports of atypical weakly haemolytic isolates of B. hyodysenteriae (whBh). In this study, 34 European whBh and 82 strongly haemolytic isolates were subjected to comparative genomic analysis. A phylogenetic tree constructed using core single nucleotide polymorphisms showed that the whBh formed a distinct sub-clade. All eight genes previously associated with haemolysis in B. hyodysenteriae were present in the whBh. No consistent patterns of amino acid substitutions for all whBh were found in these genes. In contrast, a genome region containing six coding sequences (CDSs) had consistent nucleotide sequence differences between strongly and whBh isolates. Two CDSs were predicted to encode ABC transporter proteins, and a TolC family protein, which may have a role in the export of haemolysins from B. hyodysenteriae. Another difference in this region was the presence of three CDSs in whBh that are pseudogenes in strongly haemolytic isolates. One of the intact CDSs from whBh encoded a predicted PadR-like transcriptional repressor that may play a role in repression of haemolysis functions. In summary, a sub-clade of whBh isolates has emerged in Europe, and several genomic differences, that potentially explain the weakly haemolytic phenotype, were identified. These markers may provide targets for discriminatory molecular tests needed in SD surveillance.
Details
- Title
- Weakly haemolytic variants of Brachyspira hyodysenteriae newly emerged in Europe belong to a distinct subclade with unique genetic properties
- Authors/Creators
- R.M. Card (Author/Creator) - Animal and Plant Health AgencyT. La (Author/Creator) - Murdoch UniversityE.R. Burrough (Author/Creator) - Iowa State UniversityR.J. Ellis (Author/Creator) - Animal and Plant Health AgencyJ. Nunez-Garcia (Author/Creator) - Animal and Plant Health AgencyJ.R. Thomson (Author/Creator) - Scotland's Rural CollegeM. Mahu (Author/Creator) - Ghent UniversityN.D. Phillips (Author/Creator) - Murdoch UniversityD.J. Hampson (Author/Creator) - City University of Hong KongJ. Rohde (Author/Creator) - Leibniz University HannoverA.W. Tucker (Author/Creator) - University of Cambridge
- Publication Details
- Veterinary Research Communications, Vol.50, Article number: 21
- Publisher
- Springer Netherlands
- Identifiers
- 991005542612007891
- Copyright
- © 2019 The Author(s)
- Murdoch Affiliation
- School of Veterinary and Life Sciences
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
51 File views/ downloads
31 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.248 Sexually Transmitted Infections
- 1.248.2445 Lawsonia Intracellularis
- Web Of Science research areas
- Veterinary Sciences
- ESI research areas
- Plant & Animal Science