Wheat TaMs1 is a glycosylphosphatidylinositol-anchored lipid transfer protein necessary for pollen development

Allan Kouidri; Ute Baumann; Takashi Okada; Mathieu Baes; Elise J. Tucker; Ryan Whitford

doi:10.1186/s12870-018-1557-1

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Wheat TaMs1 is a glycosylphosphatidylinositol-anchored lipid transfer protein necessary for pollen development

Journal article

Open access

Peer reviewed

Wheat TaMs1 is a glycosylphosphatidylinositol-anchored lipid transfer protein necessary for pollen development

Allan Kouidri, Ute Baumann, Takashi Okada, Mathieu Baes, Elise J. Tucker and Ryan Whitford

BMC plant biology, Vol.18(1), 332

2018

DOI: https://doi.org/10.1186/s12870-018-1557-1

PMID: 30518316

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Abstract

Wheat

LTP

Glycosylphosphatidylinositol-anchored lipid transfer protein

Sporopollenin

Pollen exine

Male sterility

Background In flowering plants, lipid biosynthesis and transport within anthers is essential for male reproductive success. TaMs1, a dominant wheat fertility gene located on chromosome 4BS, has been previously fine mapped and identified to encode a glycosylphosphatidylinositol (GPI)-anchored non-specific lipid transfer protein (nsLTP). Although this gene is critical for pollen exine development, details of its function remains poorly understood. Results In this study, we report that TaMs1 is only expressed from the B sub-genome, with highest transcript abundance detected in anthers containing microspores undergoing pre-meiosis through to meiosis. β-glucuronidase transcriptional fusions further revealed that TaMs1 is expressed throughout all anther cell-types. TaMs1 was identified to be expressed at an earlier stage of anther development relative to genes reported to be necessary for sporopollenin precursor biosynthesis. In anthers missing a functional TaMs1 (ms1c deletion mutant), these same genes were not observed to be mis-regulated, indicating an independent function for TaMs1 in pollen development. Exogenous hormone treatments on GUS reporter lines suggest that TaMs1 expression is increased by both indole-3-acetic acid (IAA) and abscisic acid (ABA). Translational fusion constructs showed that TaMs1 is targeted to the plasma membrane. Conclusions In summary, TaMs1 is a wheat fertility gene, expressed early in anther development and encodes a GPI-LTP targeted to the plasma membrane. The work presented provides a new insight into the process of wheat pollen development.

Details

Title: Wheat TaMs1 is a glycosylphosphatidylinositol-anchored lipid transfer protein necessary for pollen development
Authors/Creators: Allan Kouidri - The University of Adelaide
Ute Baumann - The University of Adelaide
Takashi Okada - The University of Adelaide
Mathieu Baes - The University of Adelaide
Elise J. Tucker - The University of Adelaide
Ryan Whitford - The University of Adelaide
Publication Details: BMC plant biology, Vol.18(1), 332
Publisher: BioMed Central Ltd unless otherwise stated. Part of Springer Nature.
Number of pages: 13
Identifiers: 991005779529007891
Murdoch Affiliation: Centre for Crop and Food Innovation
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.4 Crop Science; 3.4.159 Arabidopsis
Web Of Science research areas: Plant Sciences
ESI research areas: Plant & Animal Science