Wheat quality related differential expressions of albumins and globulins revealed by two-dimensional difference gel electrophoresis (2-D DIGE)

L. Gao; A. Wang; X. Li; K. Dong; K. Wang; R. Appels; W. Ma; Y. Yan

doi:10.1016/j.jprot.2009.09.014

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Wheat quality related differential expressions of albumins and globulins revealed by two-dimensional difference gel electrophoresis (2-D DIGE)

Journal article

Peer reviewed

Wheat quality related differential expressions of albumins and globulins revealed by two-dimensional difference gel electrophoresis (2-D DIGE)

L. Gao, A. Wang, X. Li, K. Dong, K. Wang, R. Appels, W. Ma and Y. Yan

Journal of Proteomics, Vol.73(2), pp.279-296

2009

DOI: https://doi.org/10.1016/j.jprot.2009.09.014

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Abstract

Comparative proteomics analysis offers a new approach to identify differential proteins among different wheat genotypes and developmental stages. In this study, the non-prolamin expression profiles during grain development of two common or bread wheat cultivars (Triticum aestivum L.), Jing 411 and Sunstate, with different quality properties were analyzed using two-dimensional difference gel electrophoresis (2-D DIGE). Five grain developmental stages during the post-anthesis period were sampled corresponding to the cumulative averages of daily temperatures (°C: 156 °C, 250 °C, 354 °C, 447 °C and 749.5 °C). More than 400 differential protein spots detected at one or more of the developmental stages of the two cultivars were monitored, among which 230 proteins were identified by MS. Of the identified proteins, more than 85% were enzymes possessing different physiological functions. A total of 36 differential proteins were characterized between the two varieties, which are likely to be related to wheat quality attributes. About one quarter of the proteins identified expressed in multiple spots with different pIs and molecular masses, implying certain post-translational modifications (PTMs) of proteins such as phosphorylations and glycosylations. The results provide new insights into biochemical mechanisms for grain development and quality.

Details

Title: Wheat quality related differential expressions of albumins and globulins revealed by two-dimensional difference gel electrophoresis (2-D DIGE)
Authors/Creators: L. Gao (Author/Creator) - Capital Normal University
A. Wang (Author/Creator) - Capital Normal University
X. Li (Author/Creator) - Capital Normal University
K. Dong (Author/Creator) - Capital Normal University
K. Wang (Author/Creator) - Capital Normal University
R. Appels (Author/Creator) - Agriculture and Food
W. Ma (Author/Creator) - Agriculture and Food
Y. Yan (Author/Creator) - Capital Normal University
Publication Details: Journal of Proteomics, Vol.73(2), pp.279-296
Publisher: Elsevier BV
Identifiers: 991005542720107891
Copyright: © 2009 Elsevier B.V.
Murdoch Affiliation: State Agricultural Biotechnology Centre
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.85 Food Science & Technology; 3.85.99 Cereal Starch Properties
Web Of Science research areas: Biochemical Research Methods
ESI research areas: Biology & Biochemistry