Whole genome sequencing and molecular epidemiology of paediatric Staphylococcus aureus bacteraemia

A.J. Campbell; S. Mowlaboccus; G.W. Coombs; D.A. Daley; L.S. Al Yazidi; L.K. Phuong; C. Leung; E.J. Best; R.H. Webb; L. Voss; E. Athan; P.N. Britton; P.A. Bryant; C.T. Butters; J.R. Carapetis; N.S. Ching; J. Francis; T-Y Hung; C. Nourse; S. Ojaimi; A. Tai; N. Vasilunas; B. McMullan; A.C. Bowen; C.C. Blyth

doi:10.1016/j.jgar.2022.03.012

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Whole genome sequencing and molecular epidemiology of paediatric Staphylococcus aureus bacteraemia

Journal article

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Whole genome sequencing and molecular epidemiology of paediatric Staphylococcus aureus bacteraemia

A.J. Campbell, S. Mowlaboccus, G.W. Coombs, D.A. Daley, L.S. Al Yazidi, L.K. Phuong, C. Leung, E.J. Best, R.H. Webb, L. Voss, …

Journal of Global Antimicrobial Resistance, Vol.29, pp.197-206

2022

DOI: https://doi.org/10.1016/j.jgar.2022.03.012

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Abstract

Objectives The role Staphylococcus aureus antimicrobial resistance genes and toxins play in disease severity, management and outcome in childhood is an emerging field requiring further exploration. Methods A prospective multisite study of Australian and New Zealand children hospitalised with S. aureus bacteraemia (SAB) occurred over 24 months (2017–2018). Whole genome sequencing (WGS) data were paired with clinical information from the ISAIAH cohort. Results 353 SAB isolates were sequenced; 85% methicillin-susceptible S. aureus ([MSSA], 301/353) and 15% methicillin-resistant S. aureus ([MRSA], 52/353). There were 92 sequence types (STs), most commonly ST5 (18%) and ST30 (8%), grouped into 23 clonal complexes (CCs), most frequently CC5 (21%) and CC30 (12%). MSSA comprised the majority of healthcare-associated SAB (87%, 109/125), with principal clones CC15 (48%, 11/21) and CC8 (33%, 7/21). Panton-Valentine leukocidin (PVL)-positive SAB occurred in 22% (76/353); predominantly MSSA (59%, 45/76), community-onset (92%, 70/76) infections. For community-onset SAB, the only microbiological independent predictor of poor outcomes was PVL positivity (aOR 2.6 [CI 1.0–6.2]). Conclusion From this WGS paediatric SAB data, we demonstrate the previously under-recognized role MSSA has in harbouring genetic virulence and causing healthcare-associated infections. PVL positivity was the only molecular independent predictor of poor outcomes in children. These findings underscore the need for further research to define the potential implications PVL-producing strains may have on approaches to S. aureus clinical management.

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Title: Whole genome sequencing and molecular epidemiology of paediatric Staphylococcus aureus bacteraemia
Authors/Creators: A.J. Campbell (Author/Creator) - The Kids Research Institute Australia
S. Mowlaboccus (Author/Creator) - College of Science, Health, Engineering and Education, Murdoch University, Murdoch; Department of Microbiology, PathWest Laboratory Medicine WA, Fiona Stanley Hospital, Western Australia; School of Biomedical Sciences, University of Western Australia, Nedlands.
G.W. Coombs (Author/Creator) - Fiona Stanley Hospital
D.A. Daley (Author/Creator) - Fiona Stanley Hospital
L.S. Al Yazidi (Author/Creator) - Children's Hospital at Westmead
L.K. Phuong (Author/Creator) - Royal Children's Hospital
C. Leung (Author/Creator) - Wagga Wagga Base Hospital
E.J. Best (Author/Creator) - University of Auckland
R.H. Webb (Author/Creator) - Starship Children's Health
L. Voss (Author/Creator) - Starship Children's Health
E. Athan (Author/Creator) - Deakin University
P.N. Britton (Author/Creator) - Children's Hospital at Westmead
P.A. Bryant (Author/Creator) - Royal Children's Hospital
C.T. Butters (Author/Creator) - Royal Children's Hospital
J.R. Carapetis (Author/Creator) - The Kids Research Institute Australia
N.S. Ching (Author/Creator) - Monash Children’s Hospital
J. Francis (Author/Creator) - Royal Darwin Hospital
T-Y Hung (Author/Creator) - Royal Darwin Hospital
C. Nourse (Author/Creator) - Children's Health Queensland Hospital and Health Service
S. Ojaimi (Author/Creator) - Monash University
A. Tai (Author/Creator) - Barwon Health
N. Vasilunas (Author/Creator) - Women's and Children's Hospital
B. McMullan (Author/Creator) - Sydney Children's Hospital
A.C. Bowen (Author/Creator) - The Kids Research Institute Australia
C.C. Blyth (Author/Creator) - The Kids Research Institute Australia
Publication Details: Journal of Global Antimicrobial Resistance, Vol.29, pp.197-206
Publisher: Elsevier Ltd on behalf of International Society for Antimicrobial Chemotherapy
Identifiers: 991005542523907891
Copyright: © 2022 The Authors.
Murdoch Affiliation: School of Medical, Molecular and Forensic Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Industry collaboration; Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.23 Antibiotics & Antimicrobials; 1.23.173 MRSA and VRE
Web Of Science research areas: Infectious Diseases; Pharmacology & Pharmacy
ESI research areas: Immunology