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Journal article
Whole-genome sequencing based discovery of candidate genes and diagnostic markers for seed weight in groundnut
The plant genome, Vol.16(4), e20265
2022
PMID: 36478184
Abstract
Seed weight in groundnut (Arachis hypogaea L.) has direct impact on yield as well as market price because of preference for bold seeds by consumers and industry, thereby making seed-size improvement as one of the most important objectives of groundnut breeding programs globally. Marker-based early generation selection can accelerate the process of breeding for developing large-seeded varieties. In this context, we deployed the quantitative trait locus-sequencing (QTL-seq) approach on a biparental mapping population (Chico x ICGV 02251) to identify candidate genes and develop markers for seed weight in groundnut. A total of 289.4-389.4 million reads sequencing data were generated from three libraries (ICGV 02251 and two extreme bulks) achieving 93.9-95.1% genome coverage and 8.34-9.29x average read depth. The analysis of sequencing data using QTL-seq pipeline identified five genomic regions (three on chromosome B06 and one each on chromosomes B08 and B09) for seed weight. Detailed analysis of above associated genomic regions detected 182 single-nucleotide polymorphisms (SNPs) in genic and intergenic regions, and 11 of these SNPs were nonsynonymous in the genomic regions of 10 candidate genes including Ulp proteases and BIG SEED locus genes. Kompetitive allele specific polymerase chain reaction (KASP) markers for 14 SNPs were developed, and four of these markers (snpAH0031, snpAH0033, snpAH0037, and snpAH0038) were successfully validated for deployment in breeding for large-seeded groundnut varieties.
Details
- Title
- Whole-genome sequencing based discovery of candidate genes and diagnostic markers for seed weight in groundnut
- Authors/Creators
- Sunil S. Gangurde - International Crops Research Institute for the Semi-Arid TropicsAamir W. Khan - Int Crops Res Inst Semi Arid Trop, Ctr Excellence Genom & Syst Biol CEGSB, Hyderabad 502324, IndiaPasupuleti Janila - International Crops Research Institute for the Semi-Arid TropicsMurali T. Variath - International Crops Research Institute for the Semi-Arid TropicsSurendra S. Manohar - International Crops Research Institute for the Semi-Arid TropicsPrashant Singam - Osmania UniversityAnnapurna Chitikineni - International Crops Research Institute for the Semi-Arid TropicsRajeev K. Varshney - Murdoch University, Food Futures InstituteManish K. Pandey - Int Crops Res Inst Semi Arid Trop, Ctr Excellence Genom & Syst Biol CEGSB, Hyderabad 502324, India
- Publication Details
- The plant genome, Vol.16(4), e20265
- Publisher
- Wiley Periodicals LLC on behalf of Crop Science Society of America.
- Number of pages
- 19
- Grant note
- Bill & Melinda Gates Foundation (BMGF), USA National Agricultural Science Fund (NASF) of Indian Council of Agricultural Research, India Department of Science and Technology (DST); Department of Science & Technology (India) DST-INSPIRE / Govt. of India
- Identifiers
- 991005585965807891
- Copyright
- © 2022 The Authors.
- Murdoch Affiliation
- State Agricultural Biotechnology Centre
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.96 QTL
- Web Of Science research areas
- Genetics & Heredity
- Plant Sciences
- ESI research areas
- Plant & Animal Science