Journal article
miR-15b negatively correlates with lipid metabolism in mammary epithelial cells
American Journal of Physiology-Cell Physiology, Vol.314(1), pp.C43-C52
2018
Abstract
Mammary epithelial cells are regulated by steroid hormones, growth factors, and even microRNAs. miR-15b has been found to regulate lipid metabolism in adipocytes; however, its effects on lipid metabolism in mammary epithelial cells, the cells of lipid synthesis and secretion, are as yet unknown. The main purpose of this investigation was to explore the effect of miR-15b on lipid metabolism in mammary epithelial cells, along with the underlying mechanisms. miR-15b was overexpressed or inhibited by miRNA mimics or inhibitors; subsequently, lipid formation in mammary epithelial cells, and proteins related to lipid metabolism, were investigated. Through overexpression or inhibition of miR-15b expression, the current investigation found that miR-15b downregulates lipid metabolism in mammary epithelial cells and is expressed differentially at various stages of mouse and goat mammary gland development. Inhibition of miR-15b expression increased lipid content in mammary epithelial cells through elevation of the lipid synthesis enzyme fatty acid synthetase (FASN), and overexpression of miR-15b reduced lipid content in mammary epithelial cells with decreasing levels of FASN. Moreover, the steroid hormones estradiol and progesterone decreased miR-15b expression with a subsequent increase in lipid formation in mammary epithelial cells. The expression of miR-15b was lower during lactation and negatively correlated with lipid synthesis proteins, which suggests that it may be involved in lipid synthesis and milk production. miR-15b might be a useful target for altering lipid production and milk yield.
Details
- Title
- miR-15b negatively correlates with lipid metabolism in mammary epithelial cells
- Authors/Creators
- M. Chu (Author/Creator) - Qingdao Agricultural UniversityY. Zhao (Author/Creator) - Qingdao Agricultural UniversityS. Yu (Author/Creator) - Qingdao Agricultural UniversityY. Hao (Author/Creator) - Qingdao Agricultural UniversityP. Zhang (Author/Creator) - Qingdao Agricultural UniversityY. Feng (Author/Creator) - Qingdao Agricultural UniversityH. Zhang (Author/Creator) - State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences , Beijing , People's Republic of China.D. Ma (Author/Creator) - Qingdao Agricultural UniversityJ. Liu (Author/Creator) - Qingdao Agricultural UniversityM. Cheng (Author/Creator) - Qingdao Institute of Animal Husbandry and Veterinary MedicineL. Li (Author/Creator) - Qingdao Agricultural UniversityW. Shen (Author/Creator) - Qingdao Agricultural UniversityH. Cao (Author/Creator) - Laiwu Veterinary and Livestock Administration, Laiwu, People's Republic of China.Q. Li (Author/Creator) - Laiwu Veterinary and Livestock Administration, Laiwu, People's Republic of China.L. Min (Author/Creator) - Qingdao Agricultural University
- Publication Details
- American Journal of Physiology-Cell Physiology, Vol.314(1), pp.C43-C52
- Publisher
- American Physiological Society
- Identifiers
- 991005544506507891
- Copyright
- © 2018 the American Physiological Society
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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Source: InCites
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- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.196 Micro & Long Noncoding RNA
- 1.196.68 MicroRNA in Cancer
- Web Of Science research areas
- Cell Biology
- Physiology
- ESI research areas
- Molecular Biology & Genetics