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scRNA-seq Reveals the Mechanism of Fatty Acid Desaturase 2 Mutation to Repress Leaf Growth in Peanut (Arachis hypogaea L.)21.04 MB
Published (Version of Record)CC BY V4.0, Open Access
Journal article
scRNA-seq reveals the mechanism of Fatty Acid Desaturase 2 mutation to repress leaf growth in peanut (Arachis hypogaea L.)
Cells (Basel, Switzerland), Vol.12(18), 2305
2023
Abstract
Fatty Acid Desaturase 2 (FAD2) controls the conversion of oleic acids into linoleic acids. Mutations in FAD2 not only increase the high-oleic content, but also repress the leaf growth. However, the mechanism by which FAD2 regulates the growth pathway has not been elucidated in peanut leaves with single-cell resolution. In this study, we isolated fad2 mutant leaf protoplast cells to perform single-cell RNA sequencing. Approximately 24,988 individual cells with 10,249 expressed genes were classified into five major cell types. A comparative analysis of 3495 differentially expressed genes (DEGs) in distinct cell types demonstrated that fad2 inhibited the expression of the cytokinin synthesis gene LOG in vascular cells, thereby repressing leaf growth. Further, pseudo-time trajectory analysis indicated that fad2 repressed leaf cell differentiation, and cell-cycle evidence displayed that fad2 perturbed the normal cell cycle to induce the majority of cells to drop into the S phase. Additionally, important transcription factors were filtered from the DEG profiles that connected the network involved in high-oleic acid accumulation (WRKY6), activated the hormone pathway (WRKY23, ERF109), and potentially regulated leaf growth (ERF6, MYB102, WRKY30). Collectively, our study describes different gene atlases in high-oleic and normal peanut seedling leaves, providing novel biological insights to elucidate the molecular mechanism of the high-oleic peanut-associated agronomic trait at the single-cell level.
Details
- Title
- scRNA-seq reveals the mechanism of Fatty Acid Desaturase 2 mutation to repress leaf growth in peanut (Arachis hypogaea L.)
- Authors/Creators
- Puxuan Du - Guangdong Academy of Agricultural SciencesQuanqing Deng - Guangdong Academy of Agricultural SciencesWenyi WangVanika Garg - Murdoch University, Centre for Crop and Food InnovationQing Lu - Guangdong Academy of Agricultural SciencesLu Huang - Guangdong Academy of Agricultural SciencesRunfeng Wang - Guangdong Academy of Agricultural SciencesHaifen Li - Guangdong Academy of Agricultural SciencesDongxin Huai - Oil Crops Research InstituteXiaoping Chen - Guangdong Academy of Agricultural SciencesRajeev K. Varshney - Murdoch University, Food Futures InstituteYanbin Hong - Guangdong Academy of Agricultural SciencesHao Liu - Guangdong Academy of Agricultural Sciences
- Publication Details
- Cells (Basel, Switzerland), Vol.12(18), 2305
- Publisher
- MDPI AG
- Identifiers
- 991005608868807891
- Copyright
- © 2023 by the authors
- Murdoch Affiliation
- Centre for Crop and Food Innovation; Food Futures Institute; State Agricultural Biotechnology Centre
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.159 Arabidopsis
- Web Of Science research areas
- Cell Biology
- ESI research areas
- Biology & Biochemistry