Journal article
β-carotene improves oocyte development and maturation under oxidative stress in vitro
In Vitro Cellular & Developmental Biology - Animal, Vol.55(7), pp.548-558
2019
Abstract
Recently, the mean maternal age at first birth has been continuing to increase. The decline in the age-related fertility is due to the reduction in the number and the quality of the oocyte. An elevation in intra-ovarian reactive oxygen species (ROS) is correlated with the increase in maternal age, and the oxidative stress is involved in the decline in oocyte quality. Although β-carotene, a very effective quencher of ROS, has been found to have the beneficial contribution to the ovarian development and steroidogenesis, it is unknown the effect of β-carotene on the oocyte development especially oocyte maturation. This investigation aimed to explore the beneficial contribution of β-carotene on oocyte maturation under oxidative stress and the underlying mechanism. We found that the oxidative stress induced by ROS reagent Rosup inhibited oocyte development/maturation and parthenogenetic activation which could be dramatically rescued by β-carotene (57.1 ± 4.7% vs 78.9 ± 3.8%; p < 0.05) in vitro. The underlying mechanisms include that β-carotene not only reduces ROS formation and cell apoptosis, but also it can restore actin expression, cortical granule-free domain (CGFD) formation, mitochondria homogeneous distribution, and nuclear maturation. The data suggest that β-carotene acts as a potential antioxidant in the oocyte. Therefore, the findings from this investigation provide the fundamental 7knowledge for using β-carotene as an antioxidant to improve the oocyte quality and even the ovarian function.
Details
- Title
- β-carotene improves oocyte development and maturation under oxidative stress in vitro
- Authors/Creators
- S. Yu (Author/Creator)Y. Zhao (Author/Creator)Y. Feng (Author/Creator)H. Zhang (Author/Creator)L. Li (Author/Creator)W. Shen (Author/Creator)M. Zhao (Author/Creator)L. Min (Author/Creator)
- Publication Details
- In Vitro Cellular & Developmental Biology - Animal, Vol.55(7), pp.548-558
- Publisher
- Springer New York
- Identifiers
- 991005542526807891
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 1 Clinical & Life Sciences
- 1.81 Reproductive Biology
- 1.81.339 Embryo Development
- Web Of Science research areas
- Cell Biology
- Developmental Biology
- ESI research areas
- Molecular Biology & Genetics