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Letter/Communication
Polymerase Chain Reaction using the MPB64 fragment for detection of mycobacterium tuberculosis complex DNA in suspected TB cases
International journal of medical science and public health, Vol.3(11), pp.1421-1424
2014
Abstract
Background: Various primers have been used for detection of Mycobacterium tuberculosis complex (MTBC) DNA using Polymerase chain reaction (PCR). The MPB64 gene has been demonstrated to be a highly specific target for MTBC detection.
Aims & Objectives: To detect MTBC DNA using MPB64 primers in clinical specimens of Nepalese patients suspected to have tuberculosis (TB).
Materials and Methods: DNA was extracted from 44 clinical specimens of patients suspected of having TB and MPB64 targeted PCR was performed.
Results: Bands comprising 240 base pair (bp) of MPB64 sequence were present in 15 of the 44 clinical samples, of which 4 were pulmonary and the remainders were extra-pulmonary samples. The overall positivity of MPB64 targeted PCR was 34.1%.
Conclusion: PCR targeting the MPB64 fragment has a potential of detecting the MTBC DNA and has potentially valuable clinical applications in early detection of TB in Nepal.
Details
- Title
- Polymerase Chain Reaction using the MPB64 fragment for detection of mycobacterium tuberculosis complex DNA in suspected TB cases
- Authors/Creators
- Ritambhara AryalAnil SahDeepak PaudelBishnu JoshiSunil LekhakReema RajbhandariBal Hari PoudelBijaya PantGanesh NeupaneBasant Pant
- Publication Details
- International journal of medical science and public health, Vol.3(11), pp.1421-1424
- Publisher
- BiblioMed
- Identifiers
- 991005801249407891
- Murdoch Affiliation
- Personalised Medicine Centre
- Language
- English
- Resource Type
- Letter/Communication
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