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Characterisation and cross-species utility of 20 microsatellite markers for population and forensic applications in the endangered Carnaby’s Black-cockatoo, Calyptorhynchus latirostris
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Characterisation and cross-species utility of 20 microsatellite markers for population and forensic applications in the endangered Carnaby’s Black-cockatoo, Calyptorhynchus latirostris

N.E. White, P.R. Mawson, R. Dawson, M.A. Bunce and P.B.S. Spencer
Conservation Genetics Resources, Vol.1(1), pp.341-345
Springer Verlag
2009
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Abstract

We characterise 20 microsatellite loci identified from the endangered Carnaby’s Black-cockatoo (Calyptorhynchus latirostris). The primers were tested across 40 individuals from the southwest of Western Australia and displayed between 4 and 11 alleles per locus with expected heterozygosities ranging from 53 to 87% and exclusion probabilities of C0.999. These loci will be useful in population genetic studies to facilitate conservation management decisions in addition to wildlife enforcement applications for the endangered Carnaby’s Black-cockatoo. We also tested the markers in 12 high profile and smuggled species from five genera, Cacatua, Callocephalon, Calyptorhynchus, Nymphicus and Probosciger. These species detected between 2 and 19 alleles per locus with 50–100% amplification success.

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