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Non-severe burns induce a prolonged systemic metabolic phenotype indicative of a persistent inflammatory response post-injury797.56 kB
Preprint (Author's original)CC BY-NC-ND V4.0, Open Access
Preprint
Non-severe burns induce a prolonged systemic metabolic phenotype indicative of a persistent inflammatory response post-injury
2023
Abstract
Globally, burns are a significant cause of injury that can cause substantial acute trauma as well as lead to increased incidence of chronic co-morbidity and disease. To date, research has primarily focused on the systemic response to severe injury, with little in the literature reported on impact of non-severe injuries (<15% total burn surface area; TBSA). To elucidate the metabolic consequences of non-severe burn injury, longitudinal plasma was collected from adults (n=35) who presented at hospital with a non-severe burn injury at admission, and at 6 week follow up. A cross-sectional baseline sample was also collected from non-burn control participants (n=14). Samples underwent multiplatform metabolic phenotyping using 1H nuclear magnetic resonance spectroscopy and liquid chromatography-mass spectrometry to quantify 112 lipoprotein and glycoproteins signatures and 852 lipid species from across 20 subclasses.
Multivariate data modelling (Orthogonal projection to latent structures-discriminate analysis) revealed alterations in lipoprotein and lipid metabolism when comparing baseline control to hospital admission samples, with the phenotypic signature found to be sustained at follow up. Univariate (Mann-Whitney U) testing and OPLS-DA indicated specific increases in GlycB (p-value <1.0e-4), low density lipoprotein-2 subfractions (Variable importance in projection score; VIP >6.83e-1) and monoacyglyceride (20:4)(p-value <1.0e-4) and decreases in circulating anti-inflammatory high-density lipoprotein-4 subfractions (VIP >7.75e-1), phosphatidylcholines, phosphatidylglycerols, phosphatidylinositols and phosphatidylserines.
The results indicate a persistent systemic metabolic phenotype that occurs even in cases of non-severe burn injury. The phenotype is indicative of an acute inflammatory profile which continues to be sustained post-injury, suggesting an impact on systems health beyond the site of injury. The phenotypes contained metabolic signatures consistent with chronic inflammatory states reported to have elevated incidence post-burn injury. Such phenotypic signatures may provide patient stratification opportunities, to identify individual responses to injury, personalise intervention strategies and improve acute care, reducing risk of chronic co-morbidity.
Details
- Title
- Non-severe burns induce a prolonged systemic metabolic phenotype indicative of a persistent inflammatory response post-injury
- Authors/Creators
- Monique J Ryan - Harry Perkins Institute of Medical ResearchEdward RabyLuke Whiley - Murdoch University, Centre for Computational and Systems MedicineReika Masuda - Murdoch UniversitySam L Lodge (Author) - Murdoch University, Centre for Computational and Systems MedicinePhilipp Nitschke - Murdoch UniversityGarth Maker - Murdoch University, Centre for Computational and Systems MedicineJulien Wist - Murdoch University, Centre for Computational and Systems MedicineElaine Holmes - Murdoch University, Centre for Computational and Systems MedicineFiona M Wood - The University of Western AustraliaJeremy Nicholson - Murdoch University, Health Futures InstituteMark W Fear - The University of Western AustraliaNicola Gray Dr - Murdoch University, Centre for Computational and Systems Medicine
- Identifiers
- 991005618970207891
- Murdoch Affiliation
- Australian National Phenome Centre; Centre for Computational and Systems Medicine; Health Futures Institute
- Language
- English
- Resource Type
- Preprint
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