Journal article
A systematic investigation of key factors of nucleic acid precipitation toward optimized DNA/RNA isolation
BioTechniques, Vol.68(4), pp.191-199
2020
Abstract
Nucleic acid precipitation is important for virtually all molecular biology investigations. However, despite its crucial role, a systematic study of the influence factors of nucleic acid precipitation has not been reported. In the present work, via rational experimental design, key factors of nucleic acid precipitation, including the type of nucleic acid, temperature and time of incubation, speed and time of centrifugation, volume ratio of ethanol/isopropanol to nucleic acid solution, type of cation-containing salt solution and type of coprecipitator, were comprehensively evaluated in an attempt to maximize the efficiency of nucleic acid precipitation. Our results indicate that the optimal conditions of each influence factor of nucleic acid precipitation may vary in accordance with the chemistry, structure and length of nucleic acids.
Details
- Title
- A systematic investigation of key factors of nucleic acid precipitation toward optimized DNA/RNA isolation
- Authors/Creators
- Y. Li (Author/Creator)S. Chen (Author/Creator)N. Liu (Author/Creator)L. Ma (Author/Creator)T. Wang (Author/Creator)R.N. Veedu (Author/Creator)T. Liu (Author/Creator)F. Zhang (Author/Creator)H. Zhou (Author/Creator)X. Cheng (Author/Creator)X. Jing (Author/Creator)
- Publication Details
- BioTechniques, Vol.68(4), pp.191-199
- Publisher
- Informa BioSciences
- Identifiers
- 991005545057007891
- Copyright
- © 2020 Tao Wang, Fengqiu Zhang
- Murdoch Affiliation
- Centre for Molecular Medicine and Innovative Therapeutics
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 2 Chemistry
- 2.170 Nucleic Acids Chemistry
- 2.170.877 DNA Ligand Interactions
- Web Of Science research areas
- Biochemical Research Methods
- Biochemistry & Molecular Biology
- ESI research areas
- Biology & Biochemistry