Journal article
Aeration is more important than shoot orientation when rooting lentil (Lens culinaris Medik.) cv. ‘Digger” microcuttings in vitro
In Vitro Cellular & Developmental Biology - Plant, Vol.42(2), pp.197-200
2006
Abstract
Rooting in vitro was examined for lentil nodal segments to test a recently published conclusion that shoot orientation has an effect on rooting. As is the case for many species, roots initiated and grew only at the proximal end of the microcutting regardless of its orientation. When the proximal end was in agar (a hypoxic environment) the rooting percentage was low (9–25%) even when the orientation of the microcutting was altered by inventing the culture tube. In contrast, when the proximal end of the microcutting was in an aerobic environment (from the shoot being placed upside down in agar medium or placed normally or upside down in an aerated medium), rooting percentages were higher (62–100%). Medium aeration at the proximal end of the microcutting is more important than shoot orientation for in vitro rooting of lentil microcuttings.
Details
- Title
- Aeration is more important than shoot orientation when rooting lentil (Lens culinaris Medik.) cv. ‘Digger” microcuttings in vitro
- Authors/Creators
- C. Newell (Author/Creator)D. Growns (Author/Creator)J. McComb (Author/Creator)
- Publication Details
- In Vitro Cellular & Developmental Biology - Plant, Vol.42(2), pp.197-200
- Publisher
- Springer
- Identifiers
- 991005542018507891
- Murdoch Affiliation
- School of Biological Sciences and Biotechnology
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.119 Micropropagation
- Web Of Science research areas
- Cell Biology
- Developmental Biology
- Plant Sciences
- ESI research areas
- Plant & Animal Science