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Aeration is more important than shoot orientation when rooting lentil (Lens culinaris Medik.) cv. ‘Digger” microcuttings in vitro
Journal article   Peer reviewed

Aeration is more important than shoot orientation when rooting lentil (Lens culinaris Medik.) cv. ‘Digger” microcuttings in vitro

C. Newell, D. Growns and J. McComb
In Vitro Cellular & Developmental Biology - Plant, Vol.42(2), pp.197-200
2006
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Abstract

Rooting in vitro was examined for lentil nodal segments to test a recently published conclusion that shoot orientation has an effect on rooting. As is the case for many species, roots initiated and grew only at the proximal end of the microcutting regardless of its orientation. When the proximal end was in agar (a hypoxic environment) the rooting percentage was low (9–25%) even when the orientation of the microcutting was altered by inventing the culture tube. In contrast, when the proximal end of the microcutting was in an aerobic environment (from the shoot being placed upside down in agar medium or placed normally or upside down in an aerated medium), rooting percentages were higher (62–100%). Medium aeration at the proximal end of the microcutting is more important than shoot orientation for in vitro rooting of lentil microcuttings.

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Collaboration types
Domestic collaboration
Citation topics
3 Agriculture, Environment & Ecology
3.4 Crop Science
3.4.119 Micropropagation
Web Of Science research areas
Cell Biology
Developmental Biology
Plant Sciences
ESI research areas
Plant & Animal Science
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