Journal article
Airway narrowing assessed by anatomical optical coherence tomography in vitro: dynamic airway wall morphology and function
Journal of applied physiology (1985), Vol.108(2), pp.401-411
2010
PMID: 19910337
Abstract
Regulation of airway caliber by lung volume or bronchoconstrictor stimulation is dependent on physiological, structural, and mechanical events within the airway wall, including airway smooth muscle (ASM) contraction, deformation of the mucosa and cartilage, and tensioning of elastic matrices linking wall components. Despite close association between events in the airway wall and the resulting airway caliber, these have typically been studied separately: the former primarily using histological approaches, the latter with a range of imaging modalities. We describe a new optical technique, anatomical optical coherence tomography (aOCT), which allows changes at the luminal surface (airway caliber) to be temporally related to corresponding dynamic movements within the airway wall. A fiber-optic aOCT probe was inserted into the lumen of isolated, liquid-filled porcine airways. It was used to image the response to ASM contraction induced by neural stimulation and to airway inflation and deflation. Comparisons with histology indicated that aOCT provided high-resolution images of the airway lumen including mucosal folds, the entire inner wall (mucosa and ASM), and partially the cartilaginous outer wall. Airway responses assessed by aOCT revealed several phenomena in “live” airways (i.e., not fixed) previously identified by histological investigations of fixed tissue, including a geometric relationship between ASM shortening and luminal narrowing, and sliding and bending of cartilage plates. It also provided direct evidence for distensibility of the epithelial membrane and anisotropic behavior of the airway wall. Findings suggest that aOCT can be used to relate changes in airway caliber to dynamic events in the wall of airways.
Details
- Title
- Airway narrowing assessed by anatomical optical coherence tomography in vitro: dynamic airway wall morphology and function
- Authors/Creators
- Peter B. Noble - The Kids Research Institute AustraliaAdrian R. West - Univ Western Australia, Sch Biomed Biomol & Chem Sci, Perth, WA 6009, AustraliaRobert A. McLaughlin - The University of Western AustraliaJulian J. Armstrong - The University of Western AustraliaSven Becker - The University of Western AustraliaPeter K. McFawn - The University of Western AustraliaJonathan P. Williamson - Sir Charles Gairdner HospitalPeter R. Eastwood - The University of Western AustraliaDavid R. Hillman - Sir Charles Gairdner HospitalDavid D. Sampson - The University of Western AustraliaHoward W. Mitchell - The University of Western Australia
- Publication Details
- Journal of applied physiology (1985), Vol.108(2), pp.401-411
- Publisher
- Amer Physiological Soc
- Number of pages
- 11
- Grant note
- 513921; 403915; 513854; 513704 / National Health and Medical Research Council (NHMRC) Australian; National Health and Medical Research Council (NHMRC) of Australia Raine Medical Research Foundation
- Identifiers
- 991005592655607891
- Copyright
- © 2010 the American Physiological Society
- Murdoch Affiliation
- Vice Chancellery
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 1 Clinical & Life Sciences
- 1.65 Allergy
- 1.65.44 Asthma
- Web Of Science research areas
- Physiology
- Sport Sciences
- ESI research areas
- Biology & Biochemistry