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Approaches for gene targeting and targeted gene expression in plants
Journal article   Peer reviewed

Approaches for gene targeting and targeted gene expression in plants

Amjad Masood Husaini, Zerka Rashid, Reyaz-ul Rouf Mir, Bushra Aquil and Reyazul Rouf Mir
GM crops (Austin, TX), Vol.2(3), pp.150-162
2011
PMID: 22179193

Abstract

Crops, Agricultural - genetics Crops, Agricultural - growth & development Gene Expression Regulation, Plant Gene Targeting - methods Gene Targeting - trends Genes, Plant - genetics Molecular Biology - methods Molecular Biology - trends Plant Development Plants - genetics Plants, Genetically Modified - genetics Plants, Genetically Modified - growth & development Transgenes - genetics
Transgenic science and technology are fundamental to state-of-the-art plant molecular genetics and crop improvement. The new generation of technology endeavors to introduce genes 'stably' into 'site-specific' locations and in 'single copy' without the integration of extraneous vector 'backbone' sequences or selectable markers and with a 'predictable and consistent' expression. Several similar strategies and technologies, which can push the development of 'smart' genetically modified plants with desirable attributes, as well as enhance their consumer acceptability, are discussed in this review.

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