Assessing the activity of antimicrobial peptides against common marine bacteria located in rotifer (Brachionus plicatilis) cultures

C. Woods; L. Woolley; G. Partridge; M. Chen; E.F. Haney; R.E.W. Hancock; N. Buller; A. Currie

doi:10.1007/s12602-022-09928-2

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Assessing the activity of antimicrobial peptides against common marine bacteria located in rotifer (Brachionus plicatilis) cultures

Journal article

Open access

Peer reviewed

Assessing the activity of antimicrobial peptides against common marine bacteria located in rotifer (Brachionus plicatilis) cultures

C. Woods, L. Woolley, G. Partridge, M. Chen, E.F. Haney, R.E.W. Hancock, N. Buller and A. Currie

Probiotics and Antimicrobial Proteins, Vol.14, pp.620-629

2022

DOI: https://doi.org/10.1007/s12602-022-09928-2

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Abstract

Rotifers are used as the first feed for marine fish larvae and are grown in large cultures that have high loads of organic matter and heterotrophic bacteria; these bacteria are passed on to the developing fish larvae and can potentially lead to bacterial infections. A modified minimum inhibitory concentration (MIC) protocol for antimicrobial peptides was used to determine the potency of ten antimicrobial peptides (AMPs) in artificial seawater relevant to a rotifer culture (salinity of 25‰) against common marine pathogens. All of the AMPs had antimicrobial activity against the bacterial isolates when the salt concentration was approximately zero. However, in high salt concentrations, the majority of the AMPs had an MIC value greater than 65 µg mL−1 in artificial seawater (25‰). The only exceptions were 2009 (32.5 µg mL−1) and 3002 (32.5 µg mL−1) against Vibrio rotiferianus and Tenacibaculum discolor, respectively. The selected synthetic AMPs were not effective at reducing the bacterial load in brackish salt concentrations of a typical commercial rotifer culture (25‰).

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Title: Assessing the activity of antimicrobial peptides against common marine bacteria located in rotifer (Brachionus plicatilis) cultures
Authors/Creators: C. Woods (Author/Creator) - Murdoch University
L. Woolley (Author/Creator) - Department of Primary Industries and Regional Development
G. Partridge (Author/Creator) - Murdoch University
M. Chen (Author/Creator) - Department of Primary Industries and Regional Development
E.F. Haney (Author/Creator) - University of British Columbia
R.E.W. Hancock (Author/Creator) - University of British Columbia
N. Buller (Author/Creator) - Department of Primary Industries and Regional Development
A. Currie (Author/Creator) - Murdoch University
Publication Details: Probiotics and Antimicrobial Proteins, Vol.14, pp.620-629
Publisher: Springer New York LLC
Identifiers: 991005544385607891
Murdoch Affiliation: College of Science, Health, Engineering and Education; Centre for Molecular Medicine and Innovative Therapeutics
Language: English
Resource Type: Journal article

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Collaboration types: Industry collaboration; Domestic collaboration; International collaboration
Citation topics: 2 Chemistry; 2.190 Surfactants, Lipid Bilayers & Antimicrobial Peptides; 2.190.857 Antimicrobial Peptides
Web Of Science research areas: Biotechnology & Applied Microbiology; Microbiology
ESI research areas: Microbiology