Bradyrhizobium ingae sp. nov., isolated from effective nodules of Inga laurina grown in Cerrado soil

K. da Silva; S.E. De Meyer; L.F.M. Rouws; E.N.C. Farias; M.A.O. dos Santos; G. O'Hara; J.K. Ardley; A. Willems; R.M. Pitard; J.E. Zilli

doi:10.1099/ijs.0.063727-0

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Bradyrhizobium ingae sp. nov., isolated from effective nodules of Inga laurina grown in Cerrado soil

Journal article

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Bradyrhizobium ingae sp. nov., isolated from effective nodules of Inga laurina grown in Cerrado soil

K. da Silva, S.E. De Meyer, L.F.M. Rouws, E.N.C. Farias, M.A.O. dos Santos, G. O'Hara, J.K. Ardley, A. Willems, R.M. Pitard and J.E. Zilli

International Journal of Systematic and Evolutionary Microbiology, Vol.64(Pt 10), pp.3395-3401

2014

DOI: https://doi.org/10.1099/ijs.0.063727-0

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Abstract

Root-nodule bacteria were isolated from lnga laurina (Sw.) Willd. growing in the Cerrado Amazon region, State of Roraima, Brazil. The 16S rRNA gene sequences of six strains (BR 10250(T), BR 10248, BR 10249, BR 10251, BR 10252 and BR 10253) showed low similarities with currently described species of the genus Bradyrhizobium. Phylogenetic analyses of sequences of five housekeeping genes (dnaK, gyrB, recA and rpoB) revealed Bradyrhizobium iriomotense EKO5(T) to be the closest type strain (97.4% sequence similarity or less). Chemotaxonomic data, including fatty acid profiles [with the major components C-16:0 and summed feature 8 (C-18:1 omega 6c/C-18:1 omega 7c)], the slow growth rate and carbon compound utilization patterns supported the assignment of our strains to the genus Bradyrhizobium. Results from DNA DNA hybridizations and physiological traits differentiated our strains from the closest related species of the genus Bradyrhizobium with validly published names. Sequences of symbiosis-related genes for nodulation (nodC) and nitrogen fixation (nifH) grouped together with those of B. iriomotense EKO5(T) and Bradyrhizobium sp. strains BR 6610 (used as a commercial inoculant for Inga marginata in Brazil) and TUXTLAS-10 (previously observed in Central America). Based on these data, the six strains represent a novel species, for which the name Bradyrhizobium ingae sp. nov. is proposed. The type strain is BR 10250(T) (=HAMBI 3600(T)).

Details

Title: Bradyrhizobium ingae sp. nov., isolated from effective nodules of Inga laurina grown in Cerrado soil
Authors/Creators: K. da Silva (Author/Creator) - AmeriCorps VISTA
S.E. De Meyer (Author/Creator) - Murdoch University
L.F.M. Rouws (Author/Creator) - Brazilian Agricultural Research Corporation
E.N.C. Farias (Author/Creator)
M.A.O. dos Santos (Author/Creator) - Universidade Estadual de Roraima
G. O'Hara (Author/Creator)
J.K. Ardley (Author/Creator) - Murdoch University
A. Willems (Author/Creator) - Ghent University
R.M. Pitard (Author/Creator) - Brazilian Agricultural Research Corporation
J.E. Zilli (Author/Creator) - Brazilian Agricultural Research Corporation
Publication Details: International Journal of Systematic and Evolutionary Microbiology, Vol.64(Pt 10), pp.3395-3401
Publisher: Society for General Microbiology
Identifiers: 991005542422907891
Murdoch Affiliation: Centre for Rhizobium Studies
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.97 Plant Pathology; 3.97.892 Rhizobium-Legume Symbiosis
Web Of Science research areas: Microbiology
ESI research areas: Microbiology