Journal article
Bradyrhizobium manausense sp. nov., isolated from effective nodules of Vigna unguiculata grown in Brazilian Amazonian rainforest soils
International Journal of Systematic and Evolutionary Microbiology, Vol.64(Pt 7), pp.2358-2363
2014
Abstract
Root nodule bacteria were trapped within cowpea (Vigna unguiculata) in soils with different cultivation histories collected from the Amazonian rainforest in northern Brazil. Analysis of the 16S rRNA gene sequences of six strains (BR 3351T, BR 3307, BR 3310, BR 3315, BR 3323 BR and BR 3361) isolated from cowpea nodules showed that they formed a distinct group within the genus Bradyrhizobium, which was separate from previously identified type strains. Phylogenetic analyses of three housekeeping genes (glnII, recA and rpoB) revealed that Bradyrhizobium huanghuaihaiense CCBAU 23303T was the most closely related type strain (96 % sequence similarity or lower). Chemotaxonomic data, including fatty acid profiles (predominant fatty acids being C16: 0 and summed feature 8), the slow growth rate and carbon compound utilization patterns supported the assignment of the strains to the genus Bradyrhizobium. The results of DNA-DNA hybridizations, antibiotic resistance and physiological tests differentiated these novel strains from the most closely related species of the genus Bradyrhizobium with validly published names. Symbiosis-related genes for nodulation (nodC) and nitrogen fixation (nifH) grouped the novel strains of the genus Bradyrhizobium together with Bradyrhizobium iriomotense strain EK05T, with 94 % and 96 % sequence similarity, respectively. Based on these data, these six strains represent a novel species for which the name Brabyrhizobium manausense sp. nov. (BR 3351T = HAMBI 3596T), is proposed.
Details
- Title
- Bradyrhizobium manausense sp. nov., isolated from effective nodules of Vigna unguiculata grown in Brazilian Amazonian rainforest soils
- Authors/Creators
- F.V. Silva (Author/Creator) - Universidade do Estado do Rio de JaneiroS.E. De Meyer (Author/Creator) - Murdoch UniversityJ.L. Simoes-Araujo (Author/Creator) - Brazilian Agricultural Research CorporationT.d.C. Barbe (Author/Creator)G.R. Xavier (Author/Creator) - Brazilian Agricultural Research CorporationG. O'Hara (Author/Creator)J.K. Ardley (Author/Creator) - Murdoch UniversityN.G. Rumjanek (Author/Creator) - Brazilian Agricultural Research CorporationA. Willems (Author/Creator) - Ghent UniversityJ.E. Zilli (Author/Creator) - Brazilian Agricultural Research Corporation
- Publication Details
- International Journal of Systematic and Evolutionary Microbiology, Vol.64(Pt 7), pp.2358-2363
- Publisher
- Microbiology Society
- Identifiers
- 991005544703207891
- Copyright
- © 2014 IUMS.
- Murdoch Affiliation
- Centre for Rhizobium Studies
- Language
- English
- Resource Type
- Journal article
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- Domestic collaboration
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- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.97 Plant Pathology
- 3.97.892 Rhizobium-Legume Symbiosis
- Web Of Science research areas
- Microbiology
- ESI research areas
- Microbiology