Journal article
Burkholderia dipogonis sp. nov., isolated from root nodules of Dipogon lignosus in New Zealand and Western Australia
International Journal of Systematic and Evolutionary Microbiology, Vol.65(12), pp.4716-4723
2015
Abstract
Seven strains, ICMP 19430T, ICMP 19429, ICMP 19431, WSM4637, WSM4638, WSM4639 and WSM4640, were isolated from nitrogen-fixing nodules on roots of the invasive South African legume Dipogon lignosus (subfamily Papilionoideae, tribe Phaseoleae) in New Zealand and Western Australia, and their taxonomic positions were investigated by using a polyphasic approach. All seven strains grew at 10–37 °C (optimum, 25–30 °C), at pH 4.0–9.0 (optimum, pH 6.0–7.0) and with 0–2 % (w/v) NaCl (optimum growth in the absence of NaCl). On the basis of 16S rRNA gene sequence analysis, the strains showed 99.0–99.5 % sequence similarity to the closest type strain, Burkholderia phytofirmans PsJNT, and 98.4–99.7 % sequence similarity to Burkholderia caledonica LMG 19076T. The predominant fatty acids were C18 : 1ω7c (21.0 % of the total fatty acids in strain ICMP 19430T), C16 : 0 (19.1 %), C17 : 0 cyclo (18.9 %), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c; 10.7 %) and C19 : 0 cyclo ω8c (7.5 %). The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and several uncharacterized aminophospholipids and phospholipids. The major isoprenoid quinone was Q-8 and the DNA G+C content of strain ICMP 19430T was 63.2 mol%. The DNA–DNA relatedness of the novel strains with respect to the closest neighbouring members of the genus Burkholderia was 55 % or less. On the basis of 16S rRNA and recA gene sequence similarities and chemotaxonomic and phenotypic data, these strains represent a novel symbiotic species in the genus Burkholderia, for which the name Burkholderia dipogonis sp. Nov. is proposed, with the type strain ICMP 19430T (=LMG 2841T =HAMBI 3637T).
Details
- Title
- Burkholderia dipogonis sp. nov., isolated from root nodules of Dipogon lignosus in New Zealand and Western Australia
- Authors/Creators
- S-Y Sheu (Author/Creator) - National Kaohsiung Marine UniversityM-H Chen (Author/Creator) - National Kaohsiung Marine UniversityW.Y.Y. Liu (Author/Creator) - Lincoln UniversityM. Andrews (Author/Creator) - Lincoln UniversityE.K. James (Author/Creator) - James Hutton InstituteJ.K. Ardley (Author/Creator) - Murdoch UniversityS.E. De Meyer (Author/Creator) - Murdoch UniversityT.K. James (Author/Creator) - AgResearchJ.G. Howieson (Author/Creator) - Murdoch UniversityB.G. Coutinho (Author/Creator) - International Centre for Genetic Engineering and BiotechnologyW.M Chen (Author/Creator) - National Kaohsiung Marine University
- Publication Details
- International Journal of Systematic and Evolutionary Microbiology, Vol.65(12), pp.4716-4723
- Publisher
- Society for General Microbiology
- Identifiers
- 991005541442207891
- Copyright
- © 2015 IUMS
- Murdoch Affiliation
- Centre for Rhizobium Studies
- Language
- English
- Resource Type
- Journal article
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- 3 Agriculture, Environment & Ecology
- 3.97 Plant Pathology
- 3.97.892 Rhizobium-Legume Symbiosis
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- Microbiology