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Burkholderia sprentiae sp. nov. isolated from Lebeckia ambigua root nodules
Journal article   Open access   Peer reviewed

Burkholderia sprentiae sp. nov. isolated from Lebeckia ambigua root nodules

S.E. De Meyer, M. Cnockaert, J.K. Ardley, G. Maker, R. Yates, J.G. Howieson and P. Vandamme
International Journal of Systematic and Evolutionary Microbiology, Vol.63(11), pp.3950-3957
2013
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Abstract

Seven gram-negative, rod-shaped bacteria were isolated from Lebeckia ambigua root nodules and authenticated on this host. Based on the 16S rRNA gene phylogeny, they were shown to belong to the genus Burkholderia, with the representative strain WSM5005T being most closely related to Burkholderia tuberum (98.08% sequence similarity). Additionally, these strains formed a distinct group in phylogenetic trees based on the housekeeping genes gyrB and recA. Chemotaxonomic data including fatty acid profiles and analysis of respiratory quinones supported the assignment of the strains to the genus Burkholderia. Results of DNA-DNA hybridisations, physiological and biochemical tests allowed genotypic and phenotypic differentiation of our strains from the closest validly named Burkholderia neighbour species. Therefore, these strains represent a novel species for which the name Burkholderia sprentiae sp. nov. (WSM5005T = LMG 27175T = HAMBI 3357 T), is proposed.

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Collaboration types
Domestic collaboration
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Citation topics
3 Agriculture, Environment & Ecology
3.97 Plant Pathology
3.97.892 Rhizobium-Legume Symbiosis
Web Of Science research areas
Microbiology
ESI research areas
Microbiology
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