Development of DNA aptamers targeting low-molecular-weight amyloid-β peptide aggregates in vitro

M. Chakravarthy; H. Al Shamaileh; H. Huang; R.K. Tannenberg; S. Chen; S. Worrall; P.R. Dodd; R.N. Veedu

doi:10.1039/c8cc02256a

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Development of DNA aptamers targeting low-molecular-weight amyloid-β peptide aggregates in vitro

Journal article

Peer reviewed

Development of DNA aptamers targeting low-molecular-weight amyloid-β peptide aggregates in vitro

M. Chakravarthy, H. Al Shamaileh, H. Huang, R.K. Tannenberg, S. Chen, S. Worrall, P.R. Dodd and R.N. Veedu

Chemical Communications, Vol.54(36), pp.4593-4596

2018

DOI: https://doi.org/10.1039/c8cc02256a

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Abstract

We have developed a novel functional nucleic acid aptamer to amyloid-β peptide 1–40 (Aβ1–40) and investigated its potential to detect Aβ peptide fragments in neuropathologically confirmed Alzheimer brain hippocampus tissues samples. Our results demonstrate that the aptamer candidate RNV95 could detect tetrameric/pentameric low-molecular-weight Aβ aggregates in autopsy hippocampal tissue from two neuropathologically confirmed Alzheimer disease cases. Although these are preliminary observations, detailed investigations are under way. This is the first demonstration of aptamer-Aβ binding in Alzheimer brain tissues.

Details

Title: Development of DNA aptamers targeting low-molecular-weight amyloid-β peptide aggregates in vitro
Authors/Creators: M. Chakravarthy (Author/Creator) - Murdoch University
H. Al Shamaileh (Author/Creator)
H. Huang (Author/Creator) - The University of Queensland
R.K. Tannenberg (Author/Creator) - The University of Queensland
S. Chen (Author/Creator) - Murdoch University
S. Worrall (Author/Creator) - The University of Queensland
P.R. Dodd (Author/Creator) - The University of Queensland
R.N. Veedu (Author/Creator) - Murdoch University
Publication Details: Chemical Communications, Vol.54(36), pp.4593-4596
Publisher: Royal Society of Chemistry
Identifiers: 991005544163707891
Copyright: © 2018 Royal Society of Chemistry
Murdoch Affiliation: Centre for Comparative Genomics
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 2 Chemistry; 2.145 Biosensors; 2.145.243 Nanobiosensors
Web Of Science research areas: Chemistry, Multidisciplinary
ESI research areas: Chemistry