Journal article
Development of a total flavonoids extract of Artemisia Rupestris L. via nanotechnology and its antiviral effect In Vitro
Infectious Diseases Research, Vol.1(2)
2020
Abstract
Objective: We aimed to develop a novel Artemisia rupestris L. flavonoid nano-encapsulation (AFN) preparation and evaulate its anti-hepatitis B virus (HBV) activityin vitro. Methods: First, the AFN was prepared using polylactic-co-glycolic acid (PLGA). Then, after verification of the AFN, in vitro anti-virus assays were conducted by: (1) assessing the inhibitory effect of AFN on the secretion of hepatitis B surface antigens (HBsAg), hepatitis Be-antigens (HBeAg), and the replication of HBV DNAin HepG2.2.15 cells; (2) analyzing the influence of AFN on the activation rate of NF-κB positive cells; and (3) evaluating the effect of AFN on the function of glutathione peroxidase (GSH-PX) enzymes located onthe HepG2.2.15 cell membrane. Results: Compared to the original total flavonoids extract of Artemisia rupestris L. (withoutnano-encapsulation), AFN preparation under the maximum non-toxic concentration effectively inhibited the secretion of HBsAg, HBeAg, and HBV DNA from HepG2.2.15 cells. At the same time, AFN preparation promoted not only the activation rate of NF-κB positive cells, but also antiviral GSH-PX enzyme function. In conclusion, nano-encapsulation of the flavonoids extract of Artemisia rupestris L. showed an enhanced anti-HBV effect in vitro compared to the original total flavonoids extract (without nano-encapsulation); therefore, nano-encapsulation has great potential for the development of a novel antiviral herbal medicine preparation with improved efficacy.
Details
- Title
- Development of a total flavonoids extract of Artemisia Rupestris L. via nanotechnology and its antiviral effect In Vitro
- Authors/Creators
- H. Hamulati (Author/Creator)S. Chen (Author/Creator)Z. Gu (Author/Creator)Y. Sun (Author/Creator)T. Wang (Author/Creator)
- Publication Details
- Infectious Diseases Research, Vol.1(2)
- Publisher
- TMR Publishing Group Limited
- Identifiers
- 991005544653307891
- Murdoch Affiliation
- Centre for Molecular Medicine and Innovative Therapeutics
- Language
- English
- Resource Type
- Journal article
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