Journal article
Development of nucleic acid aptamer-based lateral flow assays: A robust platform for cost-effective point-of-care diagnosis
Theranostics, Vol.11(11), pp.5174-5196
2021
Abstract
Lateral flow assay (LFA) has made a paradigm shift in the in vitro diagnosis field due to its rapid turnaround time, ease of operation and exceptional affordability. Currently used LFAs predominantly use antibodies. However, the high inter-batch variations, error margin and storage requirements of the conventional antibody-based LFAs significantly impede its applications. The recent progress in aptamer technology provides an opportunity to combine the potential of aptamer and LFA towards building a promising platform for highly efficient point-of-care device development. Over the past decades, different forms of aptamer-based LFAs have been introduced for broad applications ranging from disease diagnosis, agricultural industry to environmental sciences, especially for the detection of antibody-inaccessible small molecules such as toxins and heavy metals. But commercial aptamer-based LFAs are still not used widely compared with antibodies. In this work, by analysing the key issues of aptamer-based LFA design, including immobilization strategies, signalling methods, and target capturing approaches, we provide a comprehensive overview about aptamer-based LFA design strategies to facilitate researchers to develop optimised aptamer-based LFAs.
Details
- Title
- Development of nucleic acid aptamer-based lateral flow assays: A robust platform for cost-effective point-of-care diagnosis
- Authors/Creators
- T. Wang (Author/Creator)L. Chen (Author/Creator)A. Chikkanna (Author/Creator)S. Chen (Author/Creator)I. Brusius (Author/Creator)N. Sbuh (Author/Creator)R.N. Veedu (Author/Creator)
- Publication Details
- Theranostics, Vol.11(11), pp.5174-5196
- Publisher
- Ivyspring International Publisher
- Identifiers
- 991005542643807891
- Copyright
- ©2021 Ivyspring International Publisher.
- Murdoch Affiliation
- Centre for Molecular Medicine and Innovative Therapeutics
- Language
- English
- Resource Type
- Journal article
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- 2 Chemistry
- 2.145 Biosensors
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- Web Of Science research areas
- Medicine, Research & Experimental
- ESI research areas
- Clinical Medicine