Journal article
Distribution of airway narrowing responses across generations and at branching points, assessed in vitro by anatomical optical coherence tomography
Respiratory research, Vol.11(1), pp.9-9
2010
PMCID: PMC2824705
PMID: 20092657
Abstract
Background
Previous histological and imaging studies have shown the presence of variability in the degree of bronchoconstriction of airways sampled at different locations in the lung (i.e., heterogeneity). Heterogeneity can occur at different airway generations and at branching points in the bronchial tree. Whilst heterogeneity has been detected by previous experimental approaches, its spatial relationship either within or between airways is unknown.
Methods
In this study, distribution of airway narrowing responses across a portion of the porcine bronchial tree was determined in vitro. The portion comprised contiguous airways spanning bronchial generations (#3-11), including the associated side branches. We used a recent optical imaging technique, anatomical optical coherence tomography, to image the bronchial tree in three dimensions. Bronchoconstriction was produced by carbachol administered to either the adventitial or luminal surface of the airway. Luminal cross sectional area was measured before and at different time points after constriction to carbachol and airway narrowing calculated from the percent decrease in luminal cross sectional area.
Results
When administered to the adventitial surface, the degree of airway narrowing was progressively increased from proximal to distal generations (r = 0.80 to 0.98, P < 0.05 to 0.001). This 'serial heterogeneity' was also apparent when carbachol was administered via the lumen, though it was less pronounced. In contrast, airway narrowing was not different at side branches, and was uniform both in the parent and daughter airways.
Conclusions
Our findings demonstrate that the bronchial tree expresses intrinsic serial heterogeneity, such that narrowing increases from proximal to distal airways, a relationship that is influenced by the route of drug administration but not by structural variations accompanying branching sites.
Details
- Title
- Distribution of airway narrowing responses across generations and at branching points, assessed in vitro by anatomical optical coherence tomography
- Authors/Creators
- Peter B Noble - The Kids Research Institute AustraliaRobert A McLaughlin - The University of Western AustraliaAdrian R West - Division of Clinical Sciences, Telethon Institute for Child Health Research, (Roberts Road), Perth, , Australia Physiology, School of Biomedical, Biomolecular and Chemical Sciences, University of Western Australia, (Stirling Highway), Perth, , Australia Optical + Biomedical Engineering Laboratory, School of Electrical, Electronic and Computer Engineering, University of Western Australia, (Stirling Highway), Perth, , Australia School of Anatomy and Human Biology, University of Western Australia, (Stirling Highway), Perth, , Australia Department of Pulmonary Physiology, Sir Charles Gairdner Hospital, (Hospital Avenue), Perth, , Australia West Australian Sleep Disorders Research Institute, Sir Charles Gairdner Hospital, (Hospital Avenue), Perth, , AustraliaSven Becker - The University of Western AustraliaJulian J Armstrong - The University of Western AustraliaPeter K McFawn - The University of Western AustraliaPeter R Eastwood - Sir Charles Gairdner HospitalDavid R Hillman - Sir Charles Gairdner HospitalDavid D Sampson - The University of Western AustraliaHoward W Mitchell - The University of Western Australia
- Publication Details
- Respiratory research, Vol.11(1), pp.9-9
- Publisher
- BioMed Central
- Identifiers
- 991005591573507891
- Copyright
- © 2010 Noble et al
- Murdoch Affiliation
- Vice Chancellery
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
65 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.65 Allergy
- 1.65.44 Asthma
- Web Of Science research areas
- Respiratory System
- ESI research areas
- Clinical Medicine