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Journal article
Enhancing wheat β-glucan content through precision crossbreeding: development and evaluation of biofortified lines with improved nutritional and agronomic traits
Frontiers in genetics, Vol.16, 1532956
2025
Abstract
Introduction
To address the urgent demand for biofortified wheat enriched with health-beneficial dietary fibres such as β-glucan, this study employed meticulous crossbreeding between established wheat cultivars and the β-glucan-rich wild relative Aegilops kotschyi accession “AK-3790”.
Methods
Within this context, a derivative line encompassing a pair of 7U chromosomes from Ae. Kotschyi, denoted as 63-2-13, was identified. The presence of the 7U chromosome in this line was confirmed through comprehensive molecular marker and genomic in situ hybridization (GISH) analyses. With the aim of increasing the β-glucan content in hexaploid wheat, two distinct backcross populations were developed utilizing the 63-2-13 line as the donor parent and two separate recurrent parents (WH1105 and HD3086). These populations underwent an exact selection regimen, encompassing parent-like phenotypes, heightened yield, and robust resistance to yellow rust, meticulously tracked across successive generations until the BC2F2:3 stage.
Results and Discussion
Notably, among the outcomes, selected BC2F2:3 progenies presented remarkable increases in β-glucan levels, with a notable increase (BC2F2:3 23-5) resulting in an impressive increase in the 1.76% grain β-glucan content. Despite a discernible reduction in yield compared with their high-yielding counterparts, BC2F2:3 23-5 demonstrated a harmonious trait profile, encompassing heightened β-glucan content and moderate yellow rust resistance, thus positioning it as a compelling candidate for subsequent refinement endeavors. This research notably underscores the substantial potential of precise introgression strategies for increasing the β-glucan content in wheat, thereby underscoring the imperative of adept trait optimization to ensure both yield stability and nutritional enhancement.
Details
- Title
- Enhancing wheat β-glucan content through precision crossbreeding: development and evaluation of biofortified lines with improved nutritional and agronomic traits
- Authors/Creators
- Upendra KumarSourav PanigrahiRita GoswamiYogita SinghPriyanka BalyanPrexha KapoorSundip KumarKrishna Pal SinghFarkhandah JanReyazul Rouf Mir
- Publication Details
- Frontiers in genetics, Vol.16, 1532956
- Publisher
- FRONTIERS MEDIA SA
- Number of pages
- 9
- Grant note
- Department of Biotechnology, Govt. Of India: DBT/2020/CCSHAU/1,479
The author(s) declare that financial support was received for the research and/or publication of this article. This work was supported by the Department of Biotechnology, Govt. Of India as SRF to SP[Fellow ID: DBT/2020/CCSHAU/1,479].
- Identifiers
- 991005756542107891
- Copyright
- © 2025 Kumar, Panigrahi, Goswami, Singh, Balyan, Kapoor, Kumar, Singh, Jan and Mir
- Murdoch Affiliation
- Centre for Crop and Food Innovation
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.96 QTL
- Web Of Science research areas
- Genetics & Heredity
- ESI research areas
- Molecular Biology & Genetics