Genome-wide association analysis for pollen viability under heat stress in peanut

Hrishikesh P. Ingole; Gautam Saripalli; Zachary T. Jones; Manikannan Parthiban; Johnson Toyinbo; Sruthi Narayanan; Reyazul Rouf Mir; Sachin Rustgi

doi:10.1016/j.stress.2025.100760

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Genome-wide association analysis for pollen viability under heat stress in peanut

Journal article

Open access

Peer reviewed

Genome-wide association analysis for pollen viability under heat stress in peanut

Hrishikesh P. Ingole, Gautam Saripalli, Zachary T. Jones, Manikannan Parthiban, Johnson Toyinbo, Sruthi Narayanan, Reyazul Rouf Mir and Sachin Rustgi

Plant stress (Amsterdam), Vol.15, 100760

2025

DOI: https://doi.org/10.1016/j.stress.2025.100760

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Abstract

Peanut

Heat stress tolerance

Pollen viability

Candidate gene

Mini-core collection

Genome-wide association study

Peanut is one of the most important crops, providing nutrition and food security to millions worldwide. Pollen viability (PV) is a major determinant of yield in crops and is adversely impacted by heat stress. This study aimed to identify molecular markers associated with sustained PV under heat stress. We evaluated 72 genetically non-redundant genotypes from the U.S. peanut mini-core collection for PV under heat stress and compared them to the PV of "Georgia Green," a runner-type peanut variety used as a control. 72 genotypes were grown under optimal conditions (28/22°C day/night temperatures with a 16-hour photoperiod). Once the plants reached the five-leaf stage, heat stress was applied for two weeks by raising the daytime temperature to 38°C and the nighttime temperature to 28°C. Unopened flowers were collected and assayed for PV through in vitro pollen germination. PI200441 from Japan exhibited the highest PV, while PI504614 from Colombia showed the lowest. A genome-wide association study for PV under heat stress identified a marker on chromosome 20. Haplotype analysis revealed a 6 kb region, designated qPPVA20, containing three candidate genes, two of which (ribosomal protein and copper-transporting ATPase) showed high expression in reproductive organs. The co-localization of qPPVA20 with a previously reported QTL hotspot for heat stress-related traits makes these genes important targets for future validation. Markers associated with seed lipid compositional traits, such as behenate, arachidate, oleate, and eicosenoate content under optimal growth conditions, were also identified, with plans to investigate the impact of heat stress on these QTLs in a future study.

Details

Title: Genome-wide association analysis for pollen viability under heat stress in peanut
Authors/Creators: Hrishikesh P. Ingole
Gautam Saripalli
Zachary T. Jones
Manikannan Parthiban
Johnson Toyinbo
Sruthi Narayanan
Reyazul Rouf Mir
Sachin Rustgi
Publication Details: Plant stress (Amsterdam), Vol.15, 100760
Publisher: Elsevier B.V.
Number of pages: 10
Grant note: NIFA Hatch/Multi-state Grant: S009, 1013013 National Peanut Board: 2012637, 2014086 South Carolina Peanut Board: 2012613 Clemson University Support for Early Exploration and Development (CU SEED): 1501816
This work was supported by the NIFA Hatch/Multi-state Grant (S009 and 1013013) , National Peanut Board (Grant #2012637 and 2014086) , South Carolina Peanut Board (Grant #2012613) , and Clemson University Support for Early Exploration and Development (CU SEED) (Grant #1501816) .
Identifiers: 991005739814007891
Murdoch Affiliation: Centre for Crop and Food Innovation; School of Agricultural Sciences; Food Futures Institute
Language: English
Resource Type: Journal article

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