Journal article
In vitro evolution of chemically-modified nucleic acid aptamers: Pros and cons, and comprehensive selection strategies
RNA Biology, Vol.13(12), pp.1232-1245
2016
Abstract
Nucleic acid aptamers are single-stranded DNA or RNA oligonucleotide sequences that bind to a specific target molecule with high affinity and specificity through their ability to adopt 3-dimensional structure in solution. Aptamers have huge potential as targeted therapeutics, diagnostics, delivery agents and as biosensors. However, aptamers composed of natural nucleotide monomers are quickly degraded in vivo and show poor pharmacodynamic properties. To overcome this, chemically-modified nucleic acid aptamers are developed by incorporating modified nucleotides after or during the selection process by Systematic Evolution of Ligands by EXponential enrichment (SELEX). This review will discuss the development of chemically-modified aptamers and provide the pros and cons, and new insights on in vitro aptamer selection strategies by using chemically-modified nucleic acid libraries.
Details
- Title
- In vitro evolution of chemically-modified nucleic acid aptamers: Pros and cons, and comprehensive selection strategies
- Authors/Creators
- F. Lipi (Author/Creator)S. Chen (Author/Creator)M. Chakravarthy (Author/Creator)S. Rakesh (Author/Creator)R.N. Veedu (Author/Creator)
- Publication Details
- RNA Biology, Vol.13(12), pp.1232-1245
- Publisher
- Taylor & Francis
- Identifiers
- 991005544319907891
- Copyright
- © 2016 The Author(s)
- Murdoch Affiliation
- Centre for Comparative Genomics
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 2 Chemistry
- 2.145 Biosensors
- 2.145.243 Nanobiosensors
- Web Of Science research areas
- Biochemistry & Molecular Biology
- ESI research areas
- Biology & Biochemistry