Journal article
Incubation temperature critical to successful stimulation of in vitro zygotic embryo growth in four Australian native Cyperaceae species
Plant Cell, Tissue and Organ Culture, Vol.97(2), pp.197-202
2009
Abstract
Many species of Western Australian Cyperaceae (sedges) are vital components of the indigenous flora but commonly display low seed set, poor seed quality and intractable seed dormancy. We report the effects of incubation temperature and in vitro growth media on whole seed germination compared with extracted zygotic embryo growth in Tetraria capillaris, T. octandra, Lepidosperma drummondii and L. tenue. No germination was observed from intact whole seeds of all test species regardless of the treatment evaluated. In contrast, excised zygotic embryos of all study species exhibited significant increases in growth when cultured at 15°C compared to embryos incubated at 25°C; however, optimal media for embryo growth were genera specific. Extracted embryos of T. capillaris and T. octandra exhibited maximum percentage growth (30 and 40%, respectively) at 15°C on 1/2 MS medium with no plant growth regulators required. In the case of L. drummondii and L. tenue 1 μM thidiazuron was a necessary addition to the 1/2 MS medium resulting in 40 and 77% growth of embryos (at 15°C), respectively. Incubation of extracted embryos at 25°C (regardless of medium treatment) resulted in <10% embryo growth for T. octandra and L. tenue, while the remaining two species (L. drummondii, T. capillaris) showed no embryo growth at 25°C on any medium treatment.
Details
- Title
- Incubation temperature critical to successful stimulation of in vitro zygotic embryo growth in four Australian native Cyperaceae species
- Authors/Creators
- M. Panaia (Author/Creator) - Botanic Gardens and Parks AuthorityE. Bunn (Author/Creator) - Botanic Gardens and Parks AuthorityS.R. Turner (Author/Creator) - Botanic Gardens and Parks AuthorityJ. McComb (Author/Creator) - Murdoch University
- Publication Details
- Plant Cell, Tissue and Organ Culture, Vol.97(2), pp.197-202
- Publisher
- Kluwer Academic Publishers
- Identifiers
- 991005541078507891
- Copyright
- © 2009 Springer Science+Business Media B.V
- Murdoch Affiliation
- School of Biological Sciences and Biotechnology
- Language
- English
- Resource Type
- Journal article
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