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LOX-A4 shapes Triticum urartu gene pools and contributions to the A subgenome of polyploid wheat
Journal article   Open access   Peer reviewed

LOX-A4 shapes Triticum urartu gene pools and contributions to the A subgenome of polyploid wheat

Bei Li, Zhiyong Wang, Lingli Dong, Zhenying Dong, Kunpu Zhang, Yanpeng Wang, Reyazul Rouf Mir, Jinfang Chu, Yiwen Li, Mingtian Zhao, …
Nature communications, In Press
2026
PMID: 42014395
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Pre-proof Open Access CC BY-NC-ND V4.0

Abstract

Agricultural genetics Genome Genome-wide association studies Natural variation in plants
Lipoxygenases (LOXs) play vital roles in plant growth and defense. In this study, through genomic and molecular analyses, we discover a major LOX gene (LOX-A4) differentially expressed in Triticum urartu (Tu), the diploid progenitor of A subgenome in polyploid wheat. Compared to Tu accessions carrying wild type gene (LOX-A4W), those bearing mutant allele (LOX-A4m) show better growth but lower stress tolerance. These differences concur with a wider geographical distribution of LOX-A4m accessions than LOX-A4W materials in the Fertile Crescent. Interestingly, only mutant LOX-A4 alleles are detected in 3,516 worldwide tetraploid and hexaploid wheat lines; restoring LOX-A4W expression in common wheat inhibits growth but enhances stress tolerance. Furthermore, genome-wide identity-by-state analysis reveals that polyploid wheat A subgenome is more related to the A genome in 13 LOX-A4m Tu accessions. Thus, our work provides evidence that LOX gene variation shapes plant gene pools and their contributions to polyploid genome formation via regulating growth-defense trade-offs.

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