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Journal article
Leukocyte populations in human preterm and term breast milk identified by multicolour flow cytometry
PLoS ONE, Vol.10(8), Article e0135580
2015
Abstract
Background
Extremely preterm infants are highly susceptible to bacterial infections but breast milk provides some protection. It is unknown if leukocyte numbers and subsets in milk differ between term and preterm breast milk. This study serially characterised leukocyte populations in breast milk of mothers of preterm and term infants using multicolour flow cytometry methods for extended differential leukocyte counts in blood.
Methods
Sixty mothers of extremely preterm (<28 weeks gestational age), very preterm (28–31 wk), and moderately preterm (32–36 wk), as well as term (37–41 wk) infants were recruited. Colostrum (d2–5), transitional (d8–12) and mature milk (d26–30) samples were collected, cells isolated, and leukocyte subsets analysed using flow cytometry.
Results
The major CD45+ leukocyte populations circulating in blood were also detectable in breast milk but at different frequencies. Progression of lactation was associated with decreasing CD45+ leukocyte concentration, as well as increases in the relative frequencies of neutrophils and immature granulocytes, and decreases in the relative frequencies of eosinophils, myeloid and B cell precursors, and CD16- monocytes. No differences were observed between preterm and term breast milk in leukocyte concentration, though minor differences between preterm groups in some leukocyte frequencies were observed.
Conclusions
Flow cytometry is a useful tool to identify and quantify leukocyte subsets in breast milk. The stage of lactation is associated with major changes in milk leukocyte composition in this population. Fresh preterm breast milk is not deficient in leukocytes, but shorter gestation may be associated with minor differences in leukocyte subset frequencies in preterm compared to term breast milk.
Details
- Title
- Leukocyte populations in human preterm and term breast milk identified by multicolour flow cytometry
- Authors/Creators
- S. Trend (Author/Creator) - The University of Western AustraliaE. de Jong (Author/Creator) - The University of Western AustraliaM.L. Lloyd (Author/Creator) - The University of Western AustraliaC.H. Kok (Author/Creator) - King Edward Memorial HospitalP. Richmond (Author/Creator) - The University of Western AustraliaD.A. Doherty (Author/Creator) - The University of Western AustraliaK. Simmer (Author/Creator) - King Edward Memorial HospitalF. Kakulas (Author/Creator) - The University of Western AustraliaT. Strunk (Author/Creator) - King Edward Memorial HospitalA. Currie (Author/Creator) - The University of Western Australia
- Publication Details
- PLoS ONE, Vol.10(8), Article e0135580
- Publisher
- Public Library of Science
- Identifiers
- 991005543021407891
- Copyright
- © 2015 Trend et al.
- Murdoch Affiliation
- School of Veterinary and Life Sciences
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.72 Obstetrics & Gynecology
- 1.72.891 Breastfeeding
- Web Of Science research areas
- Pediatrics
- ESI research areas
- Clinical Medicine