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Journal article
Low-intensity repetitive magnetic stimulation lowers action potential threshold and increases spike firing in layer 5 pyramidal neurons in vitro
Neuroscience, Vol.335, pp.64-71
2016
Abstract
Repetitive transcranial magnetic stimulation (rTMS) has become a popular method of modulating neural plasticity in humans. Clinically, rTMS is delivered at high intensities to modulate neuronal excitability. While the high-intensity magnetic field can be targeted to stimulate specific cortical regions, areas adjacent to the targeted area receive stimulation at a lower intensity and may contribute to the overall plasticity induced by rTMS. We have previously shown that low-intensity rTMS induces molecular and structural plasticity in vivo, but the effects on membrane properties and neural excitability have not been investigated. Here we investigated the acute effect of low-intensity repetitive magnetic stimulation (LI-rMS) on neuronal excitability and potential changes on the passive and active electrophysiological properties of layer 5 pyramidal neurons in vitro. Whole-cell current clamp recordings were made at baseline prior to subthreshold LI-rMS (600 pulses of iTBS, n = 9 cells from 7 animals) or sham (n = 10 cells from 9 animals), immediately after stimulation, as well as 10 and 20 min post-stimulation. Our results show that LI-rMS does not alter passive membrane properties (resting membrane potential and input resistance) but hyperpolarises action potential threshold and increases evoked spike-firing frequency. Increases in spike firing frequency were present throughout the 20 min post-stimulation whereas action potential (AP) threshold hyperpolarization was present immediately after stimulation and at 20 min post-stimulation. These results provide evidence that LI-rMS alters neuronal excitability of excitatory neurons. We suggest that regions outside the targeted region of high-intensity rTMS are susceptible to neuromodulation and may contribute to rTMS-induced plasticity.
Details
- Title
- Low-intensity repetitive magnetic stimulation lowers action potential threshold and increases spike firing in layer 5 pyramidal neurons in vitro
- Authors/Creators
- A.D. Tang (Author/Creator)I. Hong (Author/Creator)L.J. Boddington (Author/Creator)A.R. Garrett (Author/Creator)S. Etherington (Author/Creator)J.N.J. Reynolds (Author/Creator)J. Rodger (Author/Creator)
- Publication Details
- Neuroscience, Vol.335, pp.64-71
- Publisher
- Elsevier BV
- Identifiers
- 991005544773107891
- Copyright
- © 2016 IBRO
- Murdoch Affiliation
- School of Veterinary and Life Sciences
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
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- Citation topics
- 1 Clinical & Life Sciences
- 1.82 Gait & Posture
- 1.82.811 Transcranial Magnetic Stimulation
- Web Of Science research areas
- Neurosciences
- ESI research areas
- Neuroscience & Behavior