Media with low osmotic pressures but adequate concentrations of nutrients for batch culture of tobacco cells

M. Dracup; J. Gibbs; J.A. McComb

doi:10.1016/S0176-1617(87)80147-5

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Media with low osmotic pressures but adequate concentrations of nutrients for batch culture of tobacco cells

Journal article

Peer reviewed

Media with low osmotic pressures but adequate concentrations of nutrients for batch culture of tobacco cells

M. Dracup, J. Gibbs and J.A. McComb

Journal of Plant Physiology, Vol.127(3-4), pp.281-291

1987

DOI: https://doi.org/10.1016/S0176-1617(87)80147-5

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Abstract

In this study we developed media for studying water relations of, and effects of salinity on batch cultured tobacco cells. The media supported exponential growth by cells throughout the culture periods and contain much lower concentrations of sugars, K+, NO3− and NH4+ than most traditional cell culture media. These nutrients are less concentrated in the medium used for experimental cultures (LNE) than in the medium used for stock cultures (LNS) and must be replenished during culture periods in the case of LNE medium. The media contain less than 45 mol m−3 of mineral nutrients and less than 60 mol m−3 glucose. Their osmotic pressure is less than 0.24 MPa and varied by less than 0.05 MPa (LNE) and 0.15MPa (LNS) during culture cycles. Growth of tobacco cells was not affected by these reductions in macronutrients and similar reductions could probably be made in other cell culture media without affecting growth rate although this would have to be verified for other species of cells.

Details

Title: Media with low osmotic pressures but adequate concentrations of nutrients for batch culture of tobacco cells
Authors/Creators: M. Dracup (Author/Creator)
J. Gibbs (Author/Creator)
J.A. McComb (Author/Creator)
Publication Details: Journal of Plant Physiology, Vol.127(3-4), pp.281-291
Publisher: Elsevier GmbH
Identifiers: 991005543113507891
Copyright: © 1987 Gustav Fischer Verlag,
Murdoch Affiliation: School of Environmental and Life Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.4 Crop Science; 3.4.119 Micropropagation
Web Of Science research areas: Plant Sciences
ESI research areas: Plant & Animal Science