Journal article
Method of bacterial killing differentially affects the human innate immune response to Staphylococcus epidermidis
Innate Immunity, Vol.17(6), pp.508-516
2011
Abstract
Background: In vitro investigations of human innate immune responses to extracellular bacteria commonly utilise killed preparations in preference to live organisms. The effects of the bacterial preparation method on the activation of innate signalling pathways by the common opportunistic pathogen Staphylococcus epidermidis (SE) are unknown.
Materials and Methods: Mononuclear cell cytokine expression patterns induced by live (LSE), heat-killed (HKSE) and ethanol-killed SE (EKSE) were characterized at the transcriptional and translational level. Toll-like receptor (TLR)-activating capacity of the preparations was analysed using TLR-transfected human embryonic kidney cells.
Results: Live SE activated NF-κB, STAT1, type I interferon, and inflammasome pathways. Killed preparations engaged the NF-κB pathway, but had significantly lower capacity to activate other innate immune pathways.
Conclusions: Killing of extracellular bacteria has significant qualitative and quantitative effects on key aspects of innate responses in vitro. Interpretation of in vitro data and extrapolation of findings should take into account the potential effects of bacterial preparation and should not assume that responses to killed bacteria are predictive of responses to live organisms.
Details
- Title
- Method of bacterial killing differentially affects the human innate immune response to Staphylococcus epidermidis
- Authors/Creators
- T. Strunk (Author/Creator) - School of Paediatrics and Child HealthP. Richmond (Author/Creator) - School of Paediatrics and Child HealthA. Prosser (Author/Creator) - School of Paediatrics and Child HealthK. Simmer (Author/Creator) - School of Women's and Infants' HealthO. Levy (Author/Creator) - Boston Children's HospitalD. Burgner (Author/Creator) - School of Paediatrics and Child HealthA.J. Currie (Author/Creator) - School of Paediatrics and Child Health
- Publication Details
- Innate Immunity, Vol.17(6), pp.508-516
- Publisher
- Sage Publications
- Identifiers
- 991005544389707891
- Copyright
- (c) The Author(s) 2010
- Murdoch Affiliation
- School of Veterinary and Biomedical Sciences
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.6 Immunology
- 1.6.609 Toll-like Receptors
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Immunology
- Medicine, Research & Experimental
- Microbiology
- ESI research areas
- Immunology