Modulation of synaptic vesicle exocytosis in muscle-dependent long-term depression at the amphibian neuromuscular junction

S.J. Etherington; V.P.A. Johnstone; A.W. Everett

doi:10.1371/journal.pone.0087174

Back

Modulation of synaptic vesicle exocytosis in muscle-dependent long-term depression at the amphibian neuromuscular junction

Journal article

Open access

Peer reviewed

Modulation of synaptic vesicle exocytosis in muscle-dependent long-term depression at the amphibian neuromuscular junction

S.J. Etherington, V.P.A. Johnstone and A.W. Everett

PLoS ONE, Vol.9(1), e87174

2014

DOI: https://doi.org/10.1371/journal.pone.0087174

Files and links (2)

pdf

synaptic_vesicle_exocytosis.pdfDownload View

Published (Version of Record)CC BY V4.0, Open Access

url

Free to Read *No subscription requiredView

Abstract

We have labeled recycling synaptic vesicles at the somatic Bufo marinus neuromuscular junction with the styryl dye FM2-10 and provide direct evidence for refractoriness of exocytosis associated with a muscle activity-dependent form of long-term depression (LTD) at this synapse. FM2-10 dye unloading experiments demonstrated that the rate of vesicle exocytosis from the release ready pool (RRP) of vesicles was more than halved in the LTD (induced by 20 min of low frequency stimulation). Recovery from LTD, observed as a partial recovery of nerve-evoked muscle twitch amplitude, was accompanied by partial recovery of the refractoriness of RRP exocytosis. Unexpectedly, paired pulse plasticity, another routinely used indicator of presynaptic forms of synaptic plasticity, was unchanged in the LTD. We conclude that the LTD induces refractoriness of the neuromuscular vesicle release machinery downstream of presynaptic calcium entry.

Details

Title: Modulation of synaptic vesicle exocytosis in muscle-dependent long-term depression at the amphibian neuromuscular junction
Authors/Creators: S.J. Etherington (Author/Creator) - The University of Western Australia
V.P.A. Johnstone (Author/Creator) - The University of Western Australia
A.W. Everett (Author/Creator) - The University of Western Australia
Publication Details: PLoS ONE, Vol.9(1), e87174
Publisher: Public Library of Science
Identifiers: 991005539984307891
Copyright: © 2014 Etherington et al
Murdoch Affiliation: School of Veterinary and Life Sciences
Language: English
Resource Type: Journal article

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites

Metrics

179 File views/ downloads

37 Record Views

3 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.96 Cell Biology; 1.96.302 Membrane Trafficking
Web Of Science research areas: Neurosciences
ESI research areas: Neuroscience & Behavior