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Journal article
Molecular analysis of human tick-bitten skin yields signatures associated with distinct spatial and temporal trajectories - A proof-of-concept study
Heliyon, Vol.10(13), e33600
2024
Abstract
Tick-associated diseases present challenges due to tridirectional interactions among host-specific responses, tick toxins and salivary proteins as well as microbes. We aimed to uncover molecular mechanisms in tick-bitten skin samples (cases) and contralateral skin samples (controls) collected simultaneously from the same participants, using spatial transcriptomics. Cases and controls analysed using NanoString GeoMx Digital Spatial Profiler identified 274 upregulated and 840 downregulated differentially expressed genes (DEGs), revealing perturbations in keratinization and immune system regulation. Samples of skin biopsies taken within 72 h post tick-bite DEGs had changes in protein metabolism and viral infection pathways as compared to samples taken 3 months post tick-bite, which instead displayed significant perturbations in several epigenetic regulatory pathways, highlighting the temporal nature of the host response following tick bites. Within-individual signatures distinguished tick-bitten samples from controls and identified between-individual signatures, offering promise for future biomarker discovery to guide prognosis and therapy.
Details
- Title
- Molecular analysis of human tick-bitten skin yields signatures associated with distinct spatial and temporal trajectories - A proof-of-concept study
- Authors/Creators
- Wenna Lee - Centre for Biosecurity and One Health, Harry Butler Institute, Murdoch University, Murdoch, WA, AustraliaRym Ben-Othman - The Kids Research Institute AustraliaPatrycja Skut - The Kids Research Institute AustraliaAmy Huey-Yi Lee - Simon Fraser UniversityAmanda D. Barbosa - Centre for Biosecurity and One Health, Harry Butler Institute, Murdoch University, Murdoch, WA, AustraliaMiles Beaman - Faculty of Health and Medical Sciences, Pathology & Laboratory Medicine, University of Western Australia, Perth, WA, AustraliaAndrew Currie - Murdoch UniversityNathan T. Harvey - Faculty of Health and Medical Sciences, Pathology & Laboratory Medicine, University of Western Australia, Perth, WA, AustraliaPrasad Kumarasinghe - School of Medicine, University of Western Australia, Crawley, WA, AustraliaRoy A. Hall - Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, QLD, AustraliaJames Potter - Australian Infectious Diseases Research Centre, School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, QLD, AustraliaStephen Graves - Barwon HealthNicholas P. West - Griffith UniversityAmanda J. Cox - School of Pharmacy and Medical Sciences, and Menzies Health Institute, Griffith University, QLD, AustraliaPeter J. Irwin - Murdoch UniversityTobias R. Kollmann - The Kids Research Institute AustraliaCharlotte L. Oskam - Centre for Biosecurity and One Health, Harry Butler Institute, Murdoch University, Murdoch, WA, Australia
- Publication Details
- Heliyon, Vol.10(13), e33600
- Publisher
- Elsevier Ltd
- Identifiers
- 991005684869607891
- Copyright
- © 2024 Published by Elsevier Ltd.
- Murdoch Affiliation
- College of Environmental and Life Sciences; Harry Butler Institute; Centre for Molecular Medicine and Innovative Therapeutics; Centre for Biosecurity and One Health; School of Veterinary Medicine; School of Medical, Molecular and Forensic Sciences
- Language
- English
- Resource Type
- Journal article
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