Journal article
Novel Burkholderia bacteria isolated from Lebeckia ambigua – A perennial suffrutescent legume of the fynbos
Soil Biology and Biochemistry, Vol.60, pp.55-64
2013
Abstract
We investigated symbiotic and physiological properties, and taxonomic position, of 23 bacterial strains isolated from Lebeckia ambigua root nodules collected from the South African fynbos region. The capacity for nodulation and nitrogen fixation on three provenances of L. ambigua was investigated for these strains together with several physiological characters, including growth rate on peat and in betonite clay, survival on polyethylene beads and pH tolerance. Additionally, the 16S rRNA gene phylogeny was determined. The root nodule bacteria isolated clustered in five different groups belonging to the genus Burkholderia, most closely related to B. caledonica, B. graminis and B. tuberum. Moreover there was a very strong influence of collection site on the taxonomy of the Burkholderia strains. The physiological characterisation revealed two promising strains, WSM4174 and WSM4184, achieved rapid growth in normal media and reached high, stable numbers in sterile peat. However, there was a worrying susceptibility to desiccation amongst these Burkholderia. Additionally, evidence was found for isolation of non-symbiotic strains from the nodule material collected in South Africa.
Details
- Title
- Novel Burkholderia bacteria isolated from Lebeckia ambigua – A perennial suffrutescent legume of the fynbos
- Authors/Creators
- J.G. Howieson (Author/Creator)S.E. De Meyer (Author/Creator)A.I. Vivas-Marfisi (Author/Creator)S Ratnayake (Author/Creator)J.K. Ardley (Author/Creator)R.J. Yates (Author/Creator)
- Publication Details
- Soil Biology and Biochemistry, Vol.60, pp.55-64
- Publisher
- Elsevier BV
- Identifiers
- 991005541884207891
- Copyright
- © 2013 Elsevier Ltd.
- Murdoch Affiliation
- Centre for Rhizobium Studies
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
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- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.97 Plant Pathology
- 3.97.892 Rhizobium-Legume Symbiosis
- Web Of Science research areas
- Soil Science
- ESI research areas
- Agricultural Sciences