Journal article
Progress, opportunity, and perspective on exosome isolation - efforts for efficient exosome-based theranostics
Theranostics, Vol.10(8), pp.3684-3707
2020
Abstract
Exosomes are small extracellular vesicles with diameters of 30-150 nm. In both physiological and pathological conditions, nearly all types of cells can release exosomes, which play important roles in cell communication and epigenetic regulation by transporting crucial protein and genetic materials such as miRNA, mRNA, and DNA. Consequently, exosome-based disease diagnosis and therapeutic methods have been intensively investigated. However, as in any natural science field, the in-depth investigation of exosomes relies heavily on technological advances. Historically, the two main technical hindrances that have restricted the basic and applied researches of exosomes include, first, how to simplify the extraction and improve the yield of exosomes and, second, how to effectively distinguish exosomes from other extracellular vesicles, especially functional microvesicles. Over the past few decades, although a standardized exosome isolation method has still not become available, a number of techniques have been established through exploration of the biochemical and physicochemical features of exosomes. In this work, by comprehensively analyzing the progresses in exosome separation strategies, we provide a panoramic view of current exosome isolation techniques, providing perspectives toward the development of novel approaches for high-efficient exosome isolation from various types of biological matrices. In addition, from the perspective of exosome-based diagnosis and therapeutics, we emphasize the issue of quantitative exosome and microvesicle separation.
Details
- Title
- Progress, opportunity, and perspective on exosome isolation - efforts for efficient exosome-based theranostics
- Authors/Creators
- D. Yang (Author/Creator) - Ningbo Medical Center Lihuili HospitalW. Zhang (Author/Creator) - Zhengzhou UniversityH. Zhang (Author/Creator) - Ningbo Medical Center Lihuili HospitalF. Zhang (Author/Creator) - Zhengzhou UniversityL. Chen (Author/Creator) - Guangdong Medical CollegeL. Ma (Author/Creator) - Henan UniversityL.M. Larcher (Author/Creator) - Murdoch UniversityS. Chen (Author/Creator) - Murdoch UniversityN. Liu (Author/Creator) - Southern Medical UniversityQ. Zhao (Author/Creator) - Wake Forest UniversityP.H.L. Tran (Author/Creator) - Deakin UniversityC. Chen (Author/Creator) - First Affiliated Hospital of Zhengzhou UniversityR.N. Veedu (Author/Creator) - Murdoch UniversityT. Wang (Author/Creator) - Murdoch University
- Publication Details
- Theranostics, Vol.10(8), pp.3684-3707
- Publisher
- Ivyspring International Publisher
- Identifiers
- 991005540028307891
- Copyright
- © 2020 The author(s)
- Murdoch Affiliation
- Centre for Molecular Medicine and Innovative Therapeutics
- Language
- English
- Resource Type
- Journal article
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Source: InCites
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Highly Cited Paper
- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.196 Micro & Long Noncoding RNA
- 1.196.1475 Exosomes
- Web Of Science research areas
- Medicine, Research & Experimental
- ESI research areas
- Clinical Medicine