Stratification of Sepsis Patients on Admission into the Intensive Care Unit According to Differential Plasma Metabolic Phenotypes

Sam Lodge; Edward Litton; Nicola Gray Dr; Monique Ryan; Oscar Millet; Mark Fear; Edward Raby; Professor Andrew Currie; Fiona Wood; Elaine Holmes; Julien Wist; Jeremy Nicholson

doi:10.1021/acs.jproteome.3c00803

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Stratification of Sepsis Patients on Admission into the Intensive Care Unit According to Differential Plasma Metabolic Phenotypes

Journal article

Open access

Peer reviewed

Stratification of Sepsis Patients on Admission into the Intensive Care Unit According to Differential Plasma Metabolic Phenotypes

Sam Lodge, Edward Litton, Nicola Gray Dr, Monique Ryan, Oscar Millet, Mark Fear, Edward Raby, Professor Andrew Currie, Fiona Wood, Elaine Holmes, …

Journal of Proteome Research, Vol.23(4), pp.1328-1340

2024

DOI: https://doi.org/10.1021/acs.jproteome.3c00803

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Abstract

ICU

sepsis

septic shock

NMR spectroscopy

mass spectrometry

pharmaco-metabonomics

plasma IVDr

metabolic phenotyping

diagnostic modeling

lipoproteins

lipids

SPC

APACHE

Delayed diagnosis of patients with sepsis or septic shock is associated with increased mortality and morbidity. UPLC-MS and NMR spectroscopy were used to measure panels of lipoproteins, lipids, biogenic amines, amino acids, and tryptophan pathway metabolites in blood plasma samples collected from 152 patients within 48 h of admission into the Intensive Care Unit (ICU) where 62 patients had no sepsis, 71 patients had sepsis, and 19 patients had septic shock. Patients with sepsis or septic shock had higher concentrations of neopterin and lower levels of HDL cholesterol and phospholipid particles in comparison to nonsepsis patients. Septic shock could be differentiated from sepsis patients based on different concentrations of 10 lipids, including significantly lower concentrations of five phosphatidylcholine species, three cholesterol esters, one dihydroceramide, and one phosphatidylethanolamine. The Supramolecular Phospholipid Composite (SPC) was reduced in all ICU patients, while the composite markers of acute phase glycoproteins were increased in the sepsis and septic shock patients within 48 h admission into ICU. We show that the plasma metabolic phenotype obtained within 48 h of ICU admission is diagnostic for the presence of sepsis and that septic shock can be differentiated from sepsis based on the lipid profile.

Details

Title: Stratification of Sepsis Patients on Admission into the Intensive Care Unit According to Differential Plasma Metabolic Phenotypes
Authors/Creators: Sam Lodge - Murdoch University, Centre for Computational and Systems Medicine
Edward Litton - Fiona Stanley Hospital
Nicola Gray Dr - Murdoch University, Centre for Computational and Systems Medicine
Monique Ryan
Oscar Millet - CIC bioGUNE
Mark Fear
Edward Raby
Professor Andrew Currie - Murdoch University, Centre for Molecular Medicine and Innovative Therapeutics
Fiona Wood - Government of Western Australia
Elaine Holmes - Murdoch University, Centre for Computational and Systems Medicine
Julien Wist - Murdoch University, Centre for Computational and Systems Medicine
Jeremy Nicholson - Murdoch University, Health Futures Institute
Publication Details: Journal of Proteome Research, Vol.23(4), pp.1328-1340
Publisher: American Chemical Society
Identifiers: 991005649469107891
Murdoch Affiliation: Centre for Computational and Systems Medicine; Health Futures Institute; Australian National Phenome Centre; Centre for Molecular Medicine and Innovative Therapeutics; School of Medical, Molecular and Forensic Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Industry collaboration; Domestic collaboration; International collaboration
Citation topics: 2 Chemistry; 2.211 Mass Spectrometry; 2.211.990 Metabolomics
Web Of Science research areas: Biochemical Research Methods
ESI research areas: Biology & Biochemistry