Journal article
Symbiotic and non-symbiotic Paraburkholderia isolated from South African Lebeckia ambigua root nodules and the description of Paraburkholderia fynbosensis sp. nov.
International Journal of Systematic and Evolutionary Microbiology, Vol.68(8), pp.2607-2614
2018
Abstract
Nine Gram-negative, rod-shaped bacteria were isolated from Lebeckia ambigua root nodules. All strains were able to nodulate and fix nitrogen with Lebeckia ambigua apart from WSM4178T, WSM4181 and WSM4182. Based on the 16S rRNA gene phylogeny, all strains were closely related to Paraburkholderia species (98.4–99.9 %), belonging to the Betaproteobacteria class and Burkholderiaceae family. According to 16S rRNA gene phylogeny the closest relative for WSM4174–WSM4177 and WSM4179–WSM4180 was Paraburkholderia tuberum (99.80–99.86 %), for WSM4178T was Paraburkholderia caledonica (98.42 %) and for WSM4181–WSM4182 was Paraburkholderia graminis (99.79 %). Analysis of the gyrB and recA housekeeping genes supported the assignment of WSM4181–WSM4182 to P. graminis and the other investigated strains could be assigned to the genus Paraburkholderia . The results of DNA–DNA hybridization, physiological and biochemical tests allowed genotypic and phenotypic differentiation of WSM4178T from the closest validly published Paraburkholderia species. However, WSM4174–WSM4177 and WSM4179–WSM4180 could not reliably be distinguished from its closest neighbour and therefore complete genome comparison was performed between WSM4176 and P. tuberum STM678T which gave ANI values of 96–97 %. Chemotaxonomic data, including fatty acid profiles and quinone data supported the assignment of the strains to the genus Paraburkholderia . On the basis of genotypic and phenotypic data one novel species, Paraburkholderia fynbosensis sp. nov. (WSM4178T=LMG 27177T=HAMBI 3356T), is proposed and the isolation of P. tuberum and P. graminis from root nodules of Lebeckia ambigua is reported.
Details
- Title
- Symbiotic and non-symbiotic Paraburkholderia isolated from South African Lebeckia ambigua root nodules and the description of Paraburkholderia fynbosensis sp. nov.
- Authors/Creators
- S.E. De Meyer (Author/Creator) - The University of Western AustraliaM. Cnockaert (Author/Creator) - Ghent UniversityL. Moulin (Author/Creator) - Université de MontpellierJ.G. Howieson (Author/Creator) - The University of Western AustraliaP. Vandamme (Author/Creator) - Ghent University
- Publication Details
- International Journal of Systematic and Evolutionary Microbiology, Vol.68(8), pp.2607-2614
- Publisher
- Society for General Microbiology
- Identifiers
- 991005540103907891
- Copyright
- © 2018 IUMS
- Murdoch Affiliation
- Centre for Rhizobium Studies
- Language
- English
- Resource Type
- Journal article
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- Domestic collaboration
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- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.97 Plant Pathology
- 3.97.892 Rhizobium-Legume Symbiosis
- Web Of Science research areas
- Microbiology
- ESI research areas
- Microbiology