Journal article
Synthesis of a morpholino nucleic acid (MNA)-Uridine phosphoramidite, and exon skipping using MNA/2′-O-Methyl mixmer antisense oligonucleotide
Molecules, Vol.21(11)
2016
Abstract
In this study, we synthesised a morpholino nucleoside-uridine (MNA-U) phosphoramidite and evaluated the potential of a MNA-modified antisense oligonucleotide (AO) sequences to induce exon 23 skipping in mdx mouse myotubes in vitro towards extending the applicability of morpholino chemistry with other nucleotide monomers. We designed, synthesised, and compared exon skipping efficiencies of 20 mer MNA-modified 2′-O-methyl RNA mixmer AO on a phosphorothioate backbone (MNA/2′-OMePS) to the corresponding fully modified 2′-O-methyl RNA AO (2′-OMePS) as a control. Our results showed that the MNA/2′-OMePS efficiently induced exon 23 skipping. As expected, the 2′-OMePS AO control yielded efficient exon 23 skipping. Under the applied conditions, both the AOs showed minor products corresponding to exon 22/23 dual exon skipping in low yield. As these are very preliminary data, more detailed studies are necessary; however, based on the preliminary results, MNA nucleotides might be useful in constructing antisense oligonucleotides.
Details
- Title
- Synthesis of a morpholino nucleic acid (MNA)-Uridine phosphoramidite, and exon skipping using MNA/2′-O-Methyl mixmer antisense oligonucleotide
- Authors/Creators
- S. Chen (Author/Creator) - Murdoch UniversityB.T. Le (Author/Creator) - Murdoch UniversityK. Rahimizadeh (Author/Creator) - Murdoch UniversityK. Shaikh (Author/Creator) - GMK Research Laboratories Pvt. Ltd., Mallapur, Hyderabad 500 076, India. khalil.shaikh@gmkresearch.com.N. Mohal (Author/Creator) - GMK Research Laboratories Pvt. Ltd., Mallapur, Hyderabad 500 076, India. narinder.mohal@gmkresearch.com.R.N. Veedu (Author/Creator) - Murdoch University
- Publication Details
- Molecules, Vol.21(11)
- Publisher
- MDPI
- Identifiers
- 991005544914507891
- Copyright
- © 2016 MDPI AG
- Murdoch Affiliation
- Centre for Comparative Genomics
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
287 File views/ downloads
112 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- International collaboration
- Citation topics
- 2 Chemistry
- 2.170 Nucleic Acids Chemistry
- 2.170.988 Oligonucleotide Modifications
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Chemistry, Multidisciplinary
- ESI research areas
- Chemistry